Wild carrot lectin was isolated and purified by chromatography on CM-cellulose and DEAE-cellulose. 采用CM-纤维素及DEAE-纤维素对南鹤虱凝集素进行分离纯化。
Objective Polysaccharide fractions F1, F2 and F3 were prepared from Porphyra haitanensis by hot-water extraction and DEAE-cellulose chromatography. 目的:坛紫菜多糖分级组分F1、F2和F3是从坛紫菜中通过热水提取及DEAE-纤维素层析得到的3个多糖组分。
By using chromatography of DEAE-Cellulose, FPLC and HPLC successively, hepatocyte growthfactor ( HGF) from human fetal liver was purified to homogeneity. 利用DEAE纤维素层析、FPLC和HPLC分离,从人胎肝中获得了纯化的特异刺激肝来源细胞DNA合成的肝细胞生长因子(HGF)。
Using DEAE-Cellulose to separate the polysaccharides from jujube Mill, five content has been acquired. 采用DEAE&纤维素离子交换柱层析法对红枣多糖进行组分分析,可得到五种多糖组分。
T1 、 T2 and T3, three major constituents of thymosin, were purified by acid extraction, heat dematuration, ultrafiltration and DEAE-cellulose chromatography form bovine thymus tissue. 小牛胸腺经酸抽提、热变性、超过滤和DEAE-C柱层析分离后,得到胸腺素的三个主要组分:T1、T2和T3。
After being extracted with hot water and precipitated with alcohol, then isolated twice by DEAE-cellulose and purified by Sephadex G-100 column chromatography, two kinds of polysaccharides& Le-2-1 and Le-2-2 were obtained from mycelia of Lentinus edodes through fermentation for 72h. 用发酵培养72h的香菇菌丝体,经热水提取,乙醇沉淀,再经二次DEAE-纤维素柱分离(Cl-型和B4O7)和SephadexG-100纯化,得到两个香菇多糖&Le-2-1,Le-2-2。
Snake muscle glyceraldehyde-3-phosphate dehydrogenase ( GAPDH) has been isolated and purified by ammonium sulphate fractionation and chromatography on DEAE-cellulose and CM-cellulose. 用硫酸铵分级、DEAE-纤维素柱层析、羧甲基纤维素柱层析分离和纯化了蛇肌GAPDH。聚丙烯酰胺凝胶电泳和醋酸纤维素薄膜电泳均为一条带。
The method involves extraction of the mucose with butanol, fractionation with acetone and then ( NH4) 2SO4, DEAE-cellulose ion-exchange column chromatography and Sephadex-gel filtration. 主要包括用正丁醇提取,丙酮和硫酸铵分级,DEAE纤维素离子交换层析和Sephadex凝胶过滤。
FHP-2P was further purified by DEAE-cellulose and Sephadex G-200 chromatography column. FHP经DEAE-纤维素和SephadexG-200柱进一步纯化得到牛舌菌多糖纯品FHP-2P。
In this paper the isolation and identification of trypsin inhibitors from porcine pancreas by CM-and DEAE-Cellulose ion exchange chromatography have been reported. 本文报告用CM和DEAE纤维素离子交换层析从猪胰脏分离出对胰蛋白酶有特异性抑制作用的抑制剂。
When ⅰ < 6.000, SOD can not be eluted out from DEAE-cellulose column. pH7.4的磷酸盐缓冲液的离子强度低于6.000时,SOD不能从DEAE-纤维素柱上洗脱下来。
Methods: The Polysaccharide was extracted by water and precipitated by ethanol. It was further purified by DEAE-cellulose and polyamide. 方法:利用水提醇沉得到粉防己粗多糖,再经DEAE纤维素和聚酰胺纯制。
Two extracellular chitinases were purified to homogeneous by ( NH4) 2SO4 precipitation, DEAE-cellulose chromatography and gel filtration. 发酵液经(NH4)2SO4沉淀、DEAE-纤维素层析和凝胶过滤分离出二种几丁质酶组分,在聚丙烯酰胺凝胶电泳图上显示出两条均一的带,并且每条带都具有几丁质酶活力。
Superoxide dismutase ( SOD) from fresh goose blood was purified by Chloroform-ethanol fractionation, acetone sedimentation and DEAE-cellulose chromatography. 新鲜鹅血经氯仿&乙醇分级分离,丙酮沉淀及DEAE纤维素层析的方法得到纯铜锌超氧化物歧化酶(Cu.Zn-SOD)。
Methods DEAE-cellulose and gel-filtration chromatography were used to isolate and purify the polysaccharides whose structures were characterized by using chemical and spectral methods. 方法采用DEAE-纤维素柱色谱和凝胶渗透色谱进行分离纯化,化学和光谱方法分析其结构特征。
The technology including polyethylene glycol precipitation, barium citrate adsorption and DEAE-cellulose chromatography were applied to prepare porcine factor V. Porcine factor V was identified by SDS-PAGE. 采用聚乙二醇沉淀、柠檬酸钡吸附、DEAE纤维素离子交换层析等方法,制备了猪凝血因子V。
All samples obtained from ion-exchange chromatography, gel filtration and DEAE-cellulose chromatography as described above were concentrated and desalted by using a new type organic absorbent. 以上经离子交换树脂、凝胶过滤及DEAE&纤维素柱层析所得样品均经一种新型吸附树脂脱盐、浓缩。
The Molecular Modification of Cysteine Proteinase Inhibitor from Wheat Seed and Its Activity A porcine plasma protease inhibitor specific for sulfhydryl protease was successfully purified by ( NH4) 2SO4 fraction precipitation, DEAE-cellulose chromatography and papain-Sepharose affinity chromatography. 小麦巯基蛋白酶抑制剂的分子修饰与其生理活性研究用(NH4)2SO4分段盐析,DEAE-纤维素离子交换和Papain&Sepharose亲和层析,从猪血浆纯化了一种巯基蛋白酶抑制剂(SUlfhydrylproteinaseinhibitor;简称SPI);
Purified polysaccharide recycle rate was93.7% and97.7 using DEAE-cellulose and composite pillar. 阴、阳离子大孔树脂混合床纯化的多糖回收率为97.7%。
MethodsThe PTPS was obtained by water extraction, alcohol precipitation, and DEAE-cellulose column. 方法应用水提、乙醇沉淀、DEAE-纤维素色谱,对PTPS进行提取并分级;
The two immunogens were used to immunize New Zealand white rabbits to produce the antibodies. The antisera were purified by acrylic acid-ammonium sulfate precipitation method and DEAE-cellulose anion-exchange chromatography to obtain the IgG fraction. 用两种免疫抗原免疫新西兰白兔,所得抗血清经辛酸-硫酸铵法沉淀和DEAE-纤维素阴离子交换层析纯化,得到纯度较高的抗体IgG。