Rapid diagnosis of bacterial infection using PCR and RFLP 采用PCR与RFLP方法快速诊断细菌感染
In present investigation, PCR RFLP diagnosis technology was first applied in center testing of breeders domestically. 作者将氟烷基因型PCRRFLP诊断技术用于种猪测定。
METHODS: The homozygous deletion was detected by PCR-RFLP in106 Chinese children with SMA, gene conversion by RFLP and subtle mutations by sequencing. 方法:对106例患者,采用PCR-RFLP检测基因缺失,RFLP筛查基因转变,并对PCR产物进行测序分析。
RFLP Analysis of Bacterium Community in Paddy Soil 水稻土细菌群落结构的RFLP分析
RFLP Analysis on Bacterial Diversity of Different Nitrogen Form in Excessive Fertilization Soil 过量施肥下不同氮肥对土壤细菌多样性影响的RFLP分析
The results of RFLP were chiefly accorded with those of numerical taxonomy and SDS-PAGE analysis. 聚类结果与数值分类及全细胞可溶性蛋白电泳分析结果基本一致。
RFLP analysis on growth differentiation factor 9 in Haimen goats 海门山羊GDF9基因的RFLP分析
RFLP Analysis of Bacteria and Ammonia-Oxidizing Bacteria in Paddy Soil 水稻土细菌和氨氧化细菌的RFLP分析
Experimental results based on backcross population indicated that the sensitivity and specificity of PCR marker were almost as same as that of RFLP marker. 回交后代的鉴定结果表明,该PCR标记的灵敏度和专化性与RFLP标记基本相同。
RFLP analysis bacterial communities of remedied chromium-contaminated soil by different methods 铬污染土壤修复过程中土壤细菌群落多样性的RFLP分析
Methods A PCR-based RFLP procedure was employed to detect the genotypes of168 family trios of Han descent population in the North of China. 采用PCR技术和限制性内切酶片段长度多态性的方法,检测中国北方汉族168个核心家系的基因型。
Both RAPD and RFLP assay revealed a genetic variance at DNA level. RAPD及RFLP分析较好地揭示了供试材料在DNA水平上的遗传变异。
Methods We measured 4 SNPs in ApoE gene by PCR RFLP in 216 individuals from 7 different minority nationalities. 方法应用PCRRFLP方法对东北地区7个少数民族群体共216个健康个体该基因中4个SNP位点进行检测。
Establishment of PCR amplification and method for the detection of MBL point mutation ( PCR-RFLP); 方法PCR扩增,测定序列并建立MBL基因点突变检测方法,即PCRRFLP方法(BanⅠ酶切);
Sequencing the mutation genes screened by RFLP. 对筛查出的突变基因进行测序分析。
RFLP and SSR biotechnology were applied to identify the markers linked to Ms gene based on BSA method. 运用RFLP、SSR技术,采用集群分析法(BSA)进行了与甘蓝显性细胞核雄性不育基因连锁分子标记及分子辅助育种的研究。
And the study and exploitation of Musa germplasm resource have been developed effectively based on isozyme analysis, RFLP method, RAPD technique and SSR technique. 而同功酶技术、RFLP技术、RAPD技术、SSR技术等的发展和有效应用,使芭蕉属种质资源研究和利用得到了长足的发展。
Prospect and problem of Pvu ⅱ-RFLP of ESR gene are also analyzed. 同时还分析了ESR基因PvuⅡ-RFLP的应用前景及其存在的问题。
Three type I markers were assayed using PCR RFLP technique. 3个Ⅰ类标记用PCRRFLP技术进行分型。
Besides, methods of PCR SSCP combined with RFLP and direct DNA sequencing are also introduced. 此外,尚有PCRSSCP+RFLP及直接DNA测序方法。
Conclusion PCR and RFLP analysis of 16S-23S rDNA spacer sequence can differentiate several mycobacterium complex quickly. 结论应用16S~23Srdna间隔区序列PCR和RFLP分析能将结核分枝杆菌复合菌群等几组常规方法难鉴定的菌群加以区别。
RFLP and PCR-RFLP Markers and Genetic and Breeding Research in Yak RFLP和PCR-RFLP标记与牦牛遗传育种研究
Analysis of PCR-RFLP can indicate transmission of HCMV infection and facilitate its molecular epidemiologic studies. PCRRFLP分析可揭示HCMV感染的传播方式,可用于病毒的分子流行病学研究。
The results were confirmed by RFLP. RFLP结果证实了此突变的存在。
The mutation was confirmed by restriction fragment length polymorphism ( RFLP). 针对所发现的突变,以限制性内切酶片段长度多态性(RFLP)分析加以验证。
Results The results of two PCR-RFLP HBV genotyping methods were coincide with that of S gene sequence. 结果两种PCRRFLP及S基因测序鉴定HBV基因型结果一致。
The mutant loading was analyzed by SSCP and RFLP based on genetic analysis system. 应用基于遗传分析仪的SSCP和RFLP方法对突变的异质状态进行检测。