Objective To evaluate the value for detecting serum anti-TB_4 antibody ( TB_4-IgG) in diagnosis of tuberculosis. 目的评价结核分支杆菌H37RV多肽(TB4)抗体对结核病的诊断价值。
Purification of monoclonal antibody against TB4 antigen of mycobacterium tuberculosis H_ ( 37) Rv Strain 人型结核杆菌H(37)Rv株菌体特异性抗原TB4单克隆抗体的纯化
This study offers certain base for the research of antigens TB4 in tuberculosis diagnosis. 上述工作为进一步研究TB4抗原在结核病诊断中的作用奠定了一定的基础。
Confocal laser scanning results showed that Tb4 protein expressed with a different location in GV stage oocytes, which divided in different groups by chromatin configuration. 3. 激光共聚焦结果表明,以染色质构型差异划分的三类发育潜力不同的GV期卵母细胞当中,Tb4蛋白的定位表达有显著差异。
From the beginning of oocytes maturation until the formation of the early blastula implantation, Tb4 protein expression showed an increasing tendency. 由卵母细胞成熟开始,直至早期着床囊胚的形成,Tb4蛋白的表达水平呈现上调性变化。
In vitro cultured early embryos, Tb4 mRNA began to increase from the zygotes stage, until the morula and blastula stages, the expression reached a peak. 在早期胚胎体外培养的过程中,Tb4mRNA从合子时期开始出现上调性表达,直到桑椹胚和囊胚,其表达量达到峰值。
This molecule expressed weekly in nucleus and cytoplasm of primary follicle oocytes. But during later stages of follicle maturation, Tb4 was increasing in cytoplasm, particularly in the nuclear membrane, while the expression was weak in nuclear. 该分子在初级卵泡的卵母细胞核、质区域呈现较弱表达,而在卵泡发育后期的卵母细胞质中表达量明显升高,同时在核膜处表达明显,而在细胞核内呈现较弱表达。
Mouse ovarian immuno-histochemistry results showed that, during follicle development, Tb4 protein expression gradually increased in the oocytes. 本实验获得的结果表明:1.小鼠卵巢免疫组织化学结果显示,伴随卵泡发育,Tb4蛋白在卵母细胞中的表达量逐渐增高。
Current researches had showed that Tb4 is an important G-actin sequestering factor in cells, which could regular the activity of G-actin in various microfilaments events. 现代研究证实了Tb4是一种重要的G-actin活性隐蔽因子,在细胞微丝活动中有着调节G-actin活性的作用。
Confocal laser scanning results also showed that, Tb4 in mouse oocytes maturation and early embryonic development process, there are differences of expression and localization. 激光共聚焦结果揭示,Tb4蛋白在小鼠卵母细胞成熟和早期胚胎发育过程中,存在差异性的表达和定位变化。