Effects of Interleukin-10 on Expression of α-Smooth Muscle Actin and Nuclear Factor-κ B in Hepatic Stellate Cells in Fibrotic Rat Liver 白细胞介素10对大鼠肝星形细胞表达α-平滑肌肌动蛋白和核因子-κB的影响
It is generally accepted that the interaction between actin and phosphorylated myosin by myosin light chain kinase ( MLCK) in a Ca2+-CaM dependent manner is the principal mechanism of regulation of smooth muscle contraction. 目前已公认的平滑肌收缩与舒张的主要调节机制是在Ca2+的参与下,由肌球蛋白轻链激酶(MLCK)催化的磷酸化肌球蛋白轻链(MLC20)与肌动蛋白的相互作用而产生收缩。
α-sarcomeric actin and cardiac Troponin T ( cTnT) immunofluorescence stain and GATA-4,α-MHC gene RT-PCR analysis were used to identify cell differentiation. 免疫细胞荧光检测α-横纹肌肌动蛋白(α-SarcomericActin)、心肌肌钙蛋白(cTnT)的表达;RT-PCR检测GATA-4、α-MHC基因表达,鉴定细胞分化。
Methods: NPY mRNA levels in SD rat testes were measured by RT-PCR semi-quantity with β-actin as internal control. 方法:用RTPCR半定量法分析SD雄性大鼠睾丸中的NPYmRNA的表达,以βactin为内参照。
Objective Suitable β-actin primes are screened and selected to avoid the interference of β-actin processed pseudogenes in the human gene expression analysis. 目的筛选合适的β-肌动蛋白(β-actin)引物以排除RNA样本中残存的DNA对人类基因表达分析的干扰。
Changes of the actin and transforming growth factor-β_1 expression in the small airways of the rat with chronic obstructive lung disease 肌动蛋白和转化生长因子-β1在大鼠试验性肺气肿发生中的作用
The real-time PCR assay developed in this study can detect β-actin in broad range with high efficiency and short time. The assay provide the basis for β-actin gene of duck as a reference gene in quantitative analysis of duck mRNA expression. 本实验建立的鸭β-actin基因实时荧光定量PCR法扩增效率高、线性范围广、检测周期短,为β-actin基因作为内参基因进行鸭功能基因与病原基因表达的定量分析奠定了基础。
The level of ADAR gene expression was expressed as ratio expression rate ( RER) of ADAR gene/ β-actin according to Computer Image analysis. ADAR基因表达水平用基因/β-肌动蛋白(-βactin)灰度比值表示。
Intrahepatic gene expression profiles and alpha-smooth muscle actin patterns in hepatitis C virus induced fibrosis 丙型肝炎病毒性肝纤维化的肝内基因表达谱与α-平滑肌肌动蛋白着色模式
Dynamic Changes of α Actin Gene Expression in the Profound Muscularis of M. Quadriceps Femoris of Rats after Exhausted Swimming 大鼠力竭性游泳前后股四头肌深层肌α-肌动蛋白基因表达的动态观察
The characteristic protein of myofibroblast is named α-smooth muscle actin ( α-SMA). α-平滑肌肌动蛋白是肌成纤维细胞的标志性蛋白。
However, there was no statistic difference in positive rate of RNA integrity, OD_ 260/ OD_ 280 ratio and β-actin gene expression level between the drug-induced labor group and non-drug induced labor group. 药物引产组及非药物引产组在分离完整RNA阳性率、胚胎组织RNA的OD260/OD280比值和胚胎组织βactin基因表达量上均无统计学差异。
β-actin Promoter Directed Expression of Human pro-UK Gene in CHO Cells β-actin启动子控制的人尿激酶原基因在CHO细胞中的表达
The cells expressed cardiomyocyte specific GATA-4 、α-MHC mRNA and α-sarcomeric actin 、 cTnT protein. The myofibers inside the cells were found by transmission electron microscopy. 分化的细胞表达心肌特异的GATA-4、α-MHCmRNA及α-SarcomericActin、cTnT蛋白,同时透射电镜观察到细胞质内有明显的肌丝。
Immunoprecipitation was used to assay the protein expression and phosphorylation of CaN and ERK1/ 2, and the protein expression of α-skeletal-actin in myocardial tissues. The mRNA expression of β-myosin heavy chain(β-MHC) was measured by RT-PCR. HE染色检测心肌组织病理变化,免疫沉淀法检测ERK1/2蛋白表达、CaN蛋白表达及磷酸化、以及αskeletalactin蛋白表达,RTPCR检测心肌球蛋白重链(βMHC)mRNA表达。
Objective: To study the effect of dexamethasone ( Dex) on the expressions of alpha-sarcomeric actin(α-SA) and α-SA mRNA in myocardium of rats with endotoxemia, and to explore the protective effect of Dex on the systolic function of rat myocardium. 目的通过观察地塞米松(Dex)对内毒素血症幼年大鼠心肌肌动蛋白(α-SA)及其mRNA表达的影响,探讨Dex对内毒素血症大鼠心肌收缩功能的保护途径。
Presence of actin in nuclei and chromosomes as well as in DNA-and histone-depleted nuclei and chromosomes of higher plants is discussed. 对高等植物细胞核和染色体以及核骨架和染色体骨架是否含有肌动蛋白等问题进行了讨论。
Objective To study the mechanism of α-spectrin and F-actin in the movement and development changes of nerve growth cones. 目的为了探讨α血影蛋白(αspectrin)和纤维型肌动蛋白(Factin)在神经生长锥激烈运动与神经生长过程中的作用机制。
Objective To establish an internal standard for quantitative mRNA measurement in cultured lens epithelial cells ( LECs), and to observe the stability of the housekeeping gene beta-actin(β-actin) mRNA in normal and glucocorticoid treated cells in vitro. 目的观察糖皮质激素作用体外培养的晶状体上皮细胞时,看家基因β肌动蛋白(βactin)mRNA表达的稳定性,建立该细胞定量mRNA研究的内部参照标准。
Interactions between purified vinculin and actin were studied by means of low shear viscometry with the falling ball viscometer and electron microscopy. 用落球粘度计测量低剪切粘度的方法研究了纽带蛋白-肌动蛋白的相互作用。
Specific antisera to Physarum actin and chicken skeletal muscle myosin have been pre-pared respectively. 制备了粘菌肌动蛋白抗血清和鸡骨骼肌肌球蛋白抗血清。
The expression of F-actin and alpha-actinin were analyzed by immunofluorescence and Leica laser scan confocal microscopy. 细胞免疫荧光法和激光共聚焦显微镜观察和测定细胞骨架分子F-肌动蛋白(actin)、α-辅肌动蛋白(actinin)的表达。
The result of RT-PCR showed that the ratio between Bax and β-actin in treated group and control was 43.9% and 68.6%, respectively. RTPCR检测显示,处理组的目标条带和内标条带的灰度比值(Baxβactin)低于对照组(分别为43.9%和68.6%);
Methods With β-actin gene as reference, the COX-2 mRNA was monitored in 30 specimens of breast cancer tissue and adjacent normal breast tissue by reverse transcription-polymerase chain reaction ( RT-PCR). 方法以βactin基因为参照,应用逆转录聚合酶链反应(RTPCR)技术,检测30例乳腺癌组织及癌旁正常腺体中COX2mRNA的表达。
β-actin is a member of the actin family of genes, which play important roles in maintaining cytoskeletal structure, cell motility, cell division, intracellular movements and contractile processes. β-actin是actin家族的一员,β-actin在维持细胞结构,细胞内运动,细胞分裂等细胞生理活动方面发挥着重要的作用。
The actin cytoskeleton remodeling and apoptosis were assessed with the same methods indicated in Part I.Results Part ⅰ: Ang ⅱ promoted podocyte apoptosis in a dose-and time-dependent manner. 结果第一部分:AngⅡ以剂量和时间依赖方式诱导MPC凋亡。