The proteins were purified by His-tag affinity purification and desalting ( AKTA Explorer 10.0). 4. 用蛋白纯化系统(AKTAexplorer10.0)对表达蛋白进行His?Tag亲和层析纯化和脱盐处理,得到纯化蛋白。
So the sperm membranes were extracted by low-osmotic lytic. Thirdly, utilizing reversed phase chromatography of AKTA Explorer 100 system, the compositions of SMPs were isolated. 所以采用低渗裂解法来提取分离精子膜。第三,利用AKTAexplorer100系统中的反相层析柱对精子膜中的蛋白组分进行分离和分析。
Based on difference of isoelectric points, AKTA with DEAE anion column were used to isolate foam protein, and protein Z and LTP were better isolated. 根据等电点不同,应用AKTA的DEAE阴离子柱分离啤酒泡沫蛋白质,蛋白质Z和LTP得到较好的分离。