Aim To establish a method using tachypleus amebocyte lysate ( TAL) for testing the bacterial endotoxin in Ginkgolide B injection. 目的探讨银杏内酯B注射液的细菌内毒素检查方法,并与热原检查法比较。
METHODS Gel clot method of tachypleus amebocyte lysate ( TAL) test was applied to detect bacterial endotoxins in Raltitrexed for injection. 目的考察注射用雷替曲塞细菌内毒素检查法的可行性。
METHODS: Inhibition and enhancement test was performed with limulus amebocyte lysate manufactured by two different companies. 方法:用两个生产厂的鲎试剂对盐酸罂粟碱氯化钠注射液进行干扰试验研究。
Objective: To introduce the situation of research on bacterial endotoxin test ( BET) for proteins and peptides with Limulus Amebocyte Lysate ( LAL) method in China. 目的:介绍国内应用鲎试剂法检测蛋白质和多肽类药品细菌内毒素的研究情况,为同行提供参考。
Tachypleus Amebocyte Lysate Test Using in Transfusion Reaction 鲎试验检测方法在输液反应中的应用
Objective: To explore the feasibility of detecting bacterial endotoxin with sensitivity test of limulus amebocyte lysate ( LAL) on Ornidazole injection. 目的:通过对鲎试剂的灵敏度试验,确定鲎法检测奥硝唑注射液细菌内毒素的可行性。
Methods: The valid concentration of Qingkailing Injection and the sensibility of the tachypleus amebocyte lysate were determined by interfering test. 方法:通过预试验和干扰试验确定清开灵注射液的有效稀释浓度和相应的鲎试剂灵敏度。
Study on the Determination of Bacterial Endotoxin In Mezlocillin Sodium for Injection By Tachypleus Amebocyte Lysate 鲎试剂法检测注射用美洛西林钠的细菌内毒素
Quantitative measurement of bacterial endotoxin by clotting time in Limulus amebocyte lysate test 细菌内毒素鲎试验成胶时间定量检测法
Objective: To discuss the feasibility of using Chinese control standard endotoxin ( CSE) and Tachypleus Amoebocyte Lysate ( TAL) to substitute the USP Reference Standard Endotoxin ( RSE) and Limulus Amebocyte Lysate ( LAL) on the bacterial endotoxins test for streptomycin sulfate. 目的:探讨用中国的细菌内毒素工作标准品(CSE)和鲎试剂(TAL)替代美国的细菌内毒素参考标准品(RSE)和鲎试剂(LAL)来检测硫酸链霉素的细菌内毒素的可行性。
In order to know the pyrogen in the disposable syringes and the repeatedly used glass syringes, Limulus Amebocyte Lysate test was used to carry out batch examination. 为了解一次性注射器和反复使用的玻璃注射器含热原情况,用鲎试剂检测法进行了批量检测。
Method According to China Pharmacopoeia 2000, inhibition or enhancement test for bacterial endotoxin test was performed with Amebocyte Lysate manufactured by two different companies. 方法:通过抑制增强实验确定双黄连注射液对鲎试验凝集反应干扰情况,并按中国药典2000版细菌内毒素检查法进行干扰评价实验。
To study the Chromogenic substrate limulus amebocyte lysate ( LAL) to detect the content of bacterial endotoxin in human albumin. 为了对显色基质法测定人血白蛋白中的内毒素含量方法进行探讨。
Removal of Endotoxin from Medicine Preparations by New Designed Affinity Membrane Cartridges and Approach to Determine the Sensitivity of Tachypleus Amebocyte Lysate 亲和膜分离器的设计、药品中内毒素的去除和鲎试剂灵敏度的测定方法研究
Results: Bacterial endotoxin test can be used to detect the endotoxin in 32 times dilute solutions of Qingkailing Injection and the sensibility of the tachypleus amebocyte lysate is 0.03 Eu/ ml. 结果:清开灵注射液经32倍稀释,可用灵敏度为0.03Eu/ml的鲎试剂进行细菌内毒素检查。
Conclusion: It is available for test of the bacterial endotoxin by diluting the oxaliplatin for injection to 2 mg/ mL in tachypleus amebocyte lysate method and it can substitute for the Rabbit Method in quality control. 结论:本品1mL含奥沙利铂2mg的供试品及其稀释液对细菌内毒素检查无干扰。可用鲎试验法代替家兔热原检查法控制奥沙利铂注射液的热原。
Methods After mixing polymyxin B ( PMB) with endotoxin in certain concentration, the neutralizing ratio of PMB to endotoxin was assessed by biosensor technique and limulus amebocyte lysate test respectively. The results from the two methods were compared. 方法将PMB与一定浓度的内毒素混合后,分别用生物传感技术和鲎试验法测定PMB中和内毒素能力的比值,同时与用生物学法检测的结果进行比较。
OBJECTIVE: To compare the results of the bacterial endotoxins test between two Limulus Amebocyte Lysate ( LAL) reagents. 目的:比较两种鲎试剂的细菌内毒素检查结果,避免判断误差。
Between Rabbit Pyrogen Test and Limulus Amebocyte Lysate Test Comparative Studies for Detecting Endotoxin in IFN and Serum Albumin 鲎试验和家兔法测定干扰素和白蛋白中内毒素的比较
METHODS The common and endotoxin specific tachypleus amebocyte lysate ( TAL) reagents were applied to detect endotoxins in MSCI qualitatively and quantitatively. 方法应用普通鲎试剂及特异性鲎试剂对样品进行内毒素的定性及定量检查。
The results obtained by the limulus amebocyte lysate test were the same with those from the biosensor technique. 鲎试验结果与生物传感器测定结果基本一致。
OBJECTIVE To approach the feasibility of replacing pyrogen test with bacterial endotoxin test ( BET) by analysing the interference of the injection to Limulus Amebocyte Lysate ( LAL). 目的:分析研究大蒜注射液对鲎试剂的干扰作用,探讨以细菌内毒素检查法取代热原检查法检测大蒜注射液的可行性。
Methods: The plasma levels of endotoxin and LBP in cirrhotic patients were determined by chromogenic substrate Limulus amebocyte lysate assay and enzyme-linked immunosorbent assay ( ELISA), respectively. 方法分别以基质显色法鲎试验和酶联免疫吸附测定(ELISA)检测肝硬化患者的血浆内毒素和LBP水平;
Selecting effective single drugs or components through the reaction of tachypleus amebocyte lysate ( TAL). 3. 通过体外鲎试剂反应,筛选有效的抗内毒素单味药或活性成分。