The anergic state of T cells was not limited by doses of SEA and there were other causes on anergic state of T cells excepting IL-2. T细胞无反应性不受SEA剂量限制,T细胞受抗原刺激不能产生IL-2不是无能状态产生的唯一原因。
The ability of anergic cells to respond to antigen and/ or exogenous recombinant mouse interleukin-2 ( rmIL-2) was tested. 无能细胞中加入刺激抗原或重组小鼠IL-2(rmIL-2),观察无能细胞的反应情况。
These results suggest that ability of proliferation of CD4+ T cell gradually decrease which restrain the response to the second stimulation and is in anergic state. 结论:CD4~+T细胞在SEB刺激后虽有增埴,但增殖后的CD4~+T细胞的增殖能力逐渐下降,对二次抗原刺激增殖受抑,即处于无能状态;
Anergic cells were phenotypically analyzed by the flow cytometry. 流式细胞仪检测无能细胞的表型。
Conclusion: The activation and proliferation of naive T cells could be induced by SEA singly, and the T cells became anergic by repeated SEA induction in vitro. 结论:SEA初次诱导小鼠脾淋巴细胞时,能显著促进T细胞的活化和增殖;经SEA多次刺激(3次)可诱导T细胞的无反应性。
As professional type 1 interferon-producing cells, Pre-DC2 have a capacity to induce an anergic state in human T cell lines. 作为体内主要的Ⅰ型干扰素(IFN)分泌细胞,Pre-DC2还具有诱导T细胞无反应性的能力。
And determined the proliferation of anergic T cells response to exogenous IL-2 and different doses of SEA. 并测定无能T细胞对加大SEA剂量和外源性IL-2的应答反应。
Methods: Anergic Tcell was induced by combination of B7-1 mAb and cyclosporin A ( CsA) in vitro, cytokine gene of anergic T cells was detected by RT-PCR. 方法:将B7-1单抗和CsA联用在体外诱致了抗原特异性T细胞无能,采用RT-PCR法检测了无能T细胞细胞因子基因的表达。
In the absence of inflammatory signals these iDCs expressing low amounts of co-stimulatory molecules migrate to the draining lymph nodes and induce anergic, apoptotic, or regulatory T cells. 当缺乏炎症因子时,这些iDCs表达较低水平的协同刺激分子,从而诱导引流淋巴结内T细胞无能、凋亡或调节性T细胞的产生。
In vivo, faint responsiveness of anergic T cells induced by SEA injections at 3 days interval and at 28 days interval could be seen respectively after 21 days and 28 days. 在体内,间隔3天组和间隔28天组中诱导的无能T细胞分别从末次注射后第21天和第28天开始出现较弱的应答反应。
The responsiveness of anergic T cells could be restored gradually after some times. 但体内外诱导的无能T细胞经过一段时间的休息,其应答反应是可以逐渐恢复的。