Results One of the 2 cases given lamivudine developed reinfection, whose serum anti-HBsAg, anti-HBe, anti-HBc, HBV DNA were positive and liver specimen immunohistochemical study showed HBsAg phenotype. 结果应用拉米夫定的2例病人,有1例发生再感染,其血清HBsAg、抗Hbe、抗HBc和HBVdna均呈阳性,肝活检免疫组织化学检测有HBsAg表达。
The genes of anti-HBsAg Fab antibody were obtained from human peripheral blood lymphocytes of volunteer with high antibody titer to HBsAg by using of phage display technology. 通过噬菌体展示技术从含高滴度抗乙肝表面抗原(HBsAg)抗体的人外周血淋巴细胞获得抗HBsAgFab抗体的轻、重链基因。
AIM: To purify and refold the inclusion body of a human anti-HBsAg scFv with a 6 × His tag, and to determine the affinity constant of the purified recombinant product. 目的:对融合6×His标签的人源抗乙型肝炎表面抗原单链抗体的包涵体进行纯化和复性,并对复性产物的抗原结合性质进行鉴定。
Objective: To construct an expression vector for anti-HBsAg ScFv and interleukin-2 fusion protein in E. 目的:构建以人抗乙肝表面抗原(HBsAg)单链抗体(ScFv)为导向作用,白细胞介素-2(IL-2)为治疗作用的融合蛋白原核表达载体pQE40/ScFv-IL-2。
Study on Expression of a Human Anti-HBsAg Single-chain Fv Antibody and Interferon Gamma in Pichia Pastoris 重组人源抗HBsAg单链抗体&干扰素γ在巴氏毕赤酵母中表达的研究
Preparation and Appraisement of Anti-HBsAg Monoclonal Antibody Immunoadsorbent Anti-HBsAg单克隆抗体免疫吸附剂的制备及评价
A human anti-HBsAg Fab with abnormal sequences cloned from phage antibody library 从人源性噬菌体抗体库分离出1株含有异常序列的抗HBsAgFab克隆
The antibody-bound membranes prepared by the three methods with anti-HBsAg was used as a model antibody and nylon net as a support. 本文报道用尼龙网作载体,用抗乙型肝炎表面抗原作模型抗体,采用三种不同方法制作抗体膜,并在理论上进行了讨论。
Methods: ( 1) human anti-HBsAg, TNF-α and keratin Fabs were expressed under the control of Ara promoter or Lac promoter, and their background expression levels were compared. 方法:(1)以Ara启动子和Lac启动子驱动抗HBS、TNF-α及角蛋白的人Fab段,比较其本底表达。
BalB/ C mice immunized with rAAV-HBsAg produced anti-HBsAg antibodies. rAAV-HBsAg免疫的BalB/C小鼠能产生表面抗体(抗-HBs抗体)。
Cloning of Human IgG Fc cDNA and Expression of Whole Human Anti-HBsAg Antibody in CHO Cells 人IgGFc基因克隆及人源抗HBsAg全分子抗体在CHO细胞中表达
The experimental study of the anti-HBsAg/ HBeAg property of gallic acid 没食子酸抗HBsAg/HBeAg的实验研究
Experimental study of anti-HBsAg Chinese medicinal herbs 中草药抑制HBsAg的实验研究
Conclusions The phab obtained possesses the specificity of anti-HBsAg property. 结论所筛选出来的phab具有抗HBsAg的特异性。
Conclusion The optimal condition of cation-exchange chromatography selected by test tube method can be applied for separation and purification of anti-HBsAg Fab fragment from E. coli. 结论试管法选择的离子交换层析的最适吸附条件用于从大肠杆菌中初步分离纯化anti-HBsAgFab切实可行。
Construction of a Pastoris expression vector for a fusion protein consisting of anti-HBsAg scfv and interferon-α 人源抗HBsAg单链抗体与α干扰素融合蛋白酵母表达载体的构建
Expression of human anti-HBsAg Fab fragment 人抗-HBsAgFab段抗体的表达人抗体Fab段天然抗体库的建立
Construction and expression of human anti-HBsAg ScFv/ tP fusion protein in E.coli and identification of its binding activity 人抗HBsAg单链抗体/鱼精蛋白截短体融合蛋白基因的构建、表达及活性鉴定
Preliminary Study on Nonspecific Combination Chromatography Purification Technology of Genetically Engineered Antibody Anti-HBsAg Fab 基因工程抗体Anti-HBsAgFab非特异组合层析纯化工艺初步研究
Study on the purification of recombinant human anti-HBsAg Fab fragment 重组人抗HBsAgFab抗体的纯化方法比较研究
AIM: To construct a fusion gene of human anti-HBsAg ScFv-Tat and to analyze the binding activity and internalization of ScFv-Tat fusion protein after its expression in E. coli. 目的:构建人抗HBsAg单链抗体Tat蛋白转导结构域(ScFvTat)融合基因,在大肠杆菌中进行表达,并分析ScFvTat融合蛋白的活性及内化情况。
Isolation and characterization of anti-HBsAg immune RNA and its mRNA 抗乙型肝炎表面抗原免疫RNA及其mRNA的分离和活性鉴定
The construction and clinical application of anti-HBsAg hybridoma cell line 抗HBsAg单克隆抗体杂交瘤的建立与临床应用
Objective: To construct the single-chain Fv gene of human anti-HBsAg and to analyse the expression of the constructed gene in eukaryotic cell and prokaryotic cell. 目的构建人抗HBsAg单链抗体基因,在真核细胞和原核细胞中进行表达,并对其表达产物的活性进行鉴定。
The animal experimental studies on the radioimmunotherapy of the hepatoma with~( 131)ⅰ-human anti-HBsAg Fab ~(131)Ⅰ标记人源抗HBsAgFab对荷人肝癌裸鼠的放射免疫治疗
Construction of single-chain Fv Gene of Anti-HBsAg and its expression in COS-7 cells 抗人HBsAg单链抗体基因的构建及其在COS-7细胞中的表达
Study on efficacy of adsorption by anti-HBsAg McAb in inactivating HBV in human plasma 抗HBsAg单抗吸附法对人血浆中HBV灭活效果的研究
Anti-HBsAg antibody was assayed by ELISA and the proliferation of spleen cells was measured by MTT. 进一步用ELISA法检测血清中抗HBsAg抗体;脾淋巴细胞特异及非特异性增殖试验。
In this study, one patient with HBsAg negative, anti-HBsAg positive but HBV DNA positive was recruited. The S gene and whole genome were cloned. A lot of different mutants were found in S gene. Its significance deserves further research. Methods: 1. 我们对一例HBsAg阴性、抗-HBs阳性而HBVdna阳性病例进行了S基因以及全基因组克隆,发现S基因中存在多种不同的变异,其意义值得深入研究。