Relationship of detection of cccDNA of hepatitis B virus and serum and pathology 乙型肝炎病毒cccDNA检测与血清学及病理诊断关系
Effects of hyperin on the cccDNA of duck hepatitis B virus and its immunological regulation 金丝桃苷对鸭乙肝病毒cccDNA清除及免疫调节作用探讨
Objective To establish a method for detecting HBV cccDNA in hepatocytes of chronic hepatitis B patients. 目的建立一种肝活检组织中HBV共价闭合环状DNA的定量检测方法。
HBsAg and HBeAg in the supernatant were detected by ELISA, HBcAg in nuclei by immunohistochemistry, HBV DNA in supernatant and cells by quantitative fluorescence PCR, and cccDNA in cultured cells by nest PCR. 应用ELISA法检测培养上清中的HBsAg和HBeAg,免疫组化法检测细胞核中的HBcAg,荧光定量PCR法检测上清和细胞中HBVdna,巢式PCR法检测细胞中cccDNA。
CccDNA plays a role in the liver disease development 共价闭合环状DNA在肝病发展中的作用
The pBR322 recombinant plasmids DNA products, which purified by phenol-extract and equilibrium centrifugation in CsCl-ethidium bromide gradients, have been shown as mainly spatial conformation of covalently closed circular DNA ( i. e. cccDNA) by agarose gel electrophoresis for homogeneity detection. 将pBR322重组质粒DNA纯化,经琼脂糖凝胶电泳鉴定其主要具共价闭合环状空间构象。
Establishment and Application of a Fluorescent Quantitative PCR Method for cccDNA of HBV HBVcccDNA荧光定量PCR检测方法的建立及初步应用
Advance in HBV cccDNA detection 乙型肝炎病毒cccDNA的临床检测研究进展
Many years study show that cccDNA plays an important role in every process of the liver disease development. 多年研究发现,cccDNA在肝病发展的每个过程中起着重要的作用。
Detection of HBV antigens and cccDNA in glomeruli of patients with HBV infection and renal disease 乙型肝炎病毒感染肾炎患者肾组织病毒抗原和复制中间体的检测及其意义
[ Objective] To study the clinical significance of serum hepatitis B virus covalently closed circular DNA ( HBV cccDNA) determination in patients with chronic HBV infection. 探讨慢性乙型肝炎病毒感染者外周血乙型肝炎病毒共价闭合环状DNA检测的临床意义。
CccDNA was all negative. 各时相cccDNA检测始终阴性。
Detection of HBV cccDNA in chronic hepatitis B patients 慢性乙型肝炎患者HBVcccDNA的检测
In addition, the current antiviral therapy can not completely remove covalently closed circular DNA ( cccDNA), and can not completely clear the hepatitis B virus. 而且,现在的抗病毒疗法都不能彻底清除共价闭合环状DNA(cccDNA),不能彻底的清除乙肝病毒。
Covalently closed circular DNA ( cccDNA), the primary template for viral replication, is a key factor for persistent infection. HBV共价闭合环状DNA(covalentlyclosedcircularDNA,cccDNA)作为HBV基因组的原始复制模板,是HBV持续感染的关键因素。