Methods To calculate the bacterial endotoxin limit of cefalotin sodium for injection. 方法计算注射用头孢噻吩钠的细菌内毒素限值,通过干扰试验,确定其最大无干扰浓度。
Determination of the Related Substances in Cefalotin Sodium by HPLC 高效液相色谱法测定头孢噻吩钠中的有关物质
METHODS: Cefalotin sodium for injection was diluted in water for determination of bacterial endotoxin and allocated to sample negative and sample positive controls to determine its maximum noninterference concentration; 方法:将注射用头孢噻吩钠分别以细菌内毒素检查用水稀释成供试品阴性、阳性对照系列,以考察其最大不干扰浓度;
In the MS~ n spectra of positive ion mode of cefuroxime, cefalotin sodium, cefoselis and cefoxitin sodium, a neutral loss of CO happens at the side chain of C-7. 在电喷雾正离子模式下,头孢噻吩钠、头孢噻利、头孢西丁钠多级串联质谱中均失去C-7位侧链上一分子CO。
The maximum noninterference concentration of cefalotin sodium for injection is 1.0 mg · mL~ (-1) Conclusion The bacterial endotoxin test method can be used for the controlling of cefalotin sodium for injection. mL-1。结论注射用头孢噻吩钠可用细菌内毒素检查法控制热原。
RESULTS: All the chief peaks of cefalotin sodium and those of their related substances could be well isolated in all the specificity tests. 结果:各项专属性试验中,头孢噻吩钠主峰与其它杂质峰均分离较好;
Medicine sensitivity test showed that some pathogenic bacteria of Vibrionaceae had phenomenon of drug resistance, especially, the resistance rate to amoxicillin was 100%, and the resistance rates of aeromonas sobria and Non_01 Vibrio cholerae to cefalotin and cefoxitin were 100%. 药敏试验结果表明这些弧菌科细菌存在一定的耐药现象,特别是对羟氨苄青霉素100%耐药,且嗜水气单胞菌、非O1群霍乱弧菌对头孢噻吩、头孢西丁也100%耐药。