Isolation of human liver arginase isoenzyme fractions by chromatofocusing 聚焦层析分离人肝精氨酸酶同工酶组分
Isolation of Isoforms of Apolipoprotein C ⅲ from Human Serum by Chromatofocusing 聚焦层析法分离人血清载脂蛋白CⅢ亚型
Extracellular phytase produced by Aspergillus awamori using technique of semi-solid cultivation was purified to homogeneity by employing an initial ultrafiltration step, followed by chromatography using anion exchange, gel filtration and chromatofocusing steps. 本研究以半固体发酵方式培养泡盛曲霉(Aspergillusawamori)生产植酸酶,通过超滤、阴离子交换层析,凝胶层析等步骤,纯化了植酸酶蛋白,分子量约为118kDa。