However, globular proteins that contain multiple disulfide bonds often must be heated longer at higher temperatures to denature them. 但是含多个二硫键的球蛋白却必须在更高的温度下加热更长的时间才能使之变性。
SsDNA purification enhanced the fragments recovering from sliver stained denature PAGE gel 单链DNA纯化对变性PAGE凝胶银染片段回收效率的提高
Of course, one basis for heat processing is to denature and inactivate enzymes so that the food is not subjected to continuing enzymes activity. 当然,食品热处理的理论根据之一就是使食品中的酶变性和钝化,从而使食品不再继续受到酶的作用。
Denature gradient gel electrophoresis of stratal bacteria activation in oilfield under different pressure conditions 不同压力条件下油藏内源细菌群落激活过程中变性梯度凝胶电泳分析
Proteins perform their function at a range of their growth temperature and excessively high or low temperature will make them denature and lose their function. 每个蛋白质都有最适宜发挥其功能的温度,过高或过低的温度都会使得蛋白质变性而丧失其功能。
When most proteins are heated, they denature and lose their effectiveness. 多数的蛋白质在接受加热时,它们会变性,并且失去效用。
Rnase is rich in pancreas and small intestine and may easily denature RNA. 胰腺和小肠组织RNA酶含量丰富,极易降解RNA。
A detergent used to denature proteins and cover them in uniform charge before gel electrophoresis. 一种在凝胶电泳前用来使蛋白质发生变性并用统一的电荷覆盖蛋白质的去污剂。
When these stabilizing interactions are disrupted, the molecules unfold or denature and typically lose biological activity. 当这些稳定作用因素被破坏时,分子就会展开或变性,并且通常失去了它的生物活性。
We have compared differences between three methods of remove hemoglobin out bovine blood, and think it better to sediment by copper salt ( Cu2+) and heat denature. 对牛血Cu,Zn-SOD提取中除血红蛋白的3种不同方法进行了比较研究,其中铜盐沉淀加热变性法处理效果较为理想。
For the preparation of soybean peptides, the double-enzyme hydrolyzed separately in steps and mixed double-enzyme hydrolyzed simultaneously were both studied. The sequence of adding enzyme in the former and whether to denature the first enzyme before adding the second one were also discussed. 研究了双酶分步水解和双酶混合同步水解制备大豆多肽的效果,对双酶分步作用的加酶顺序以及在加第二种酶之前是否需要使第一种酶失活进行了讨论。
First pasteurization of the raw juice may inactivate or denature polyphenoloxidase ( PPO), so in the following processing and storage, accumulation of brown color is mainly due to Maillard reaction. 苹果浓缩汁加工单元操作中前巴氏杀菌杀处理能够钝化PPO的活性,在前巴氏杀菌后的加工单元操作及贮存过程中引起果汁褐变的主要原因是美拉德反应。
Method ( 1): increasing denature temperature to 97 ℃; 方法1:提高变性温度至97℃;
Biding activity of ScFv was evaluated by ELISA and immunohistochemical methods after its denature and renature treatment. 表达产物经变性及复性后,用免疫组化方法检测其活性。
TNF-d genotype was analyzed by DHPLC ( denature high performance liquid chromatography) and DNA sequencing. 获取受者及其同胞供者的DNA,通过变性高效液相色谱(DHPLC)法结合DNA测序检测TNF-d基因型。
Main parameters and process in both dehulling system was compared, the results show that the energy is saved and the protein function denature in low grade in hot dehulling process because of heating once. 比较了等级豆粕两种脱皮工艺特点及参数,认为热脱皮因只一次加热节约能源,对豆粕蛋白变性影响较小。
Kidney tumescent and bleeding 、 granular denature; The indications of operatiOn are: ① persistent hematuria. 肾肿胀、出血、颗粒样变性;手术指征:①肾持续性出血;
After denature and renature procedure in Ni~ ( 2+)_NTA affinity chromatography column, soluble chitinase was obtained. 在Ni2+-NTA亲和柱上经变、复性和纯化,得到可溶的几丁质酶。
MMP-1 can denature and degrade fibrillar collagens, especially type I collagen and type III collagen in the certain site of them. MMP-1能在胶原分子的特异位点裂解胶原纤维,尤其是Ⅰ型和Ⅲ型。
Obtain the crude enzyme solution by thermally denature earthworm tissue homogenate. Acquire the crude enzyme by ammonium sulfate fractional precipitation and 80% acetone precipitation. Purify the crude enzyme by ion exchange, hydrophobic exchange and gel filtration chromatography. 2. 双胸蚓组织匀浆后通过热变性获得粗酶液;利用硫酸铵分级沉淀和80%丙酮沉淀获得核酸酶的粗品;粗品依次通过离子交换层析、疏水交换层析和凝胶过滤层析进行精细纯化。
Formaldehyde can denature protein through combination with amino group in protein, and disrupt metabolism of cell, cause severe harm to human cells and cancer [ 20, 21]. 甲醛能与蛋白质中的氨基结合,使蛋白质变性,扰乱细胞的代谢,对人体细胞具有极大的破坏作用,可致癌[20,21]。
Before the purification, the protein which express in inclusion bodies required to denature ( eg guanidine hydrochloride, urea, etc.) fully dissolved under the action of the denaturant. 在包涵体表达的蛋白纯化之前需要在变性剂(如盐酸胍,尿素等)的作用下,充分溶解。
The heat treatment during processing these products will inevitably denature the major components of whey protein, such as bovine α-lactalbumin, β-lactoglobulin, lactoferrin, and so on. The degree of denaturation depends on the temperature and heat time. 而这些热处理过程不可避免地会导致乳清蛋白主要功能性组分α-乳白蛋白、β-乳球蛋白和乳铁蛋白等发生变性,其变性程度在很大程度上依赖于热处理的时间和温度。
In addition, high salt concentrations tend to reduce the solubility and denature enzyme proteins through disruption of the tertiary protein structure which is essential for enzymatic activity. 此外,高浓度的盐可降低酶蛋白的溶解性,盐也能破坏蛋白质的三级结构而使酶蛋白变性,而蛋白质的三级结构是酶的活动性所必需的。
DSC was used to measure the denature enthalpy and denature temperature of 7S and 11S globulin of different solid content. 应用DSC对不同固形物含量的大豆蛋白的7S球蛋白和11S球蛋白的变性热焓、变性温度进行了测定。
As the temperature of heating rising, abalones core temperatures are rising too. Most of the proteins begin to denature, collagen which change into dissolved gelatin losses with water. 随着加热温度的升高,鲍鱼中心温度随之升高,大部分的蛋白质开始变性,胶原蛋白变成可溶性的明胶随水分一起流失。
However, organic solvents often give rise to denature of lipase and decreased activity. Therefore, screening of organic solvent-tolerant lipases with high activity in the presence of organic solvents has become a focus of researches on lipase. 然而有机溶剂会使酶变性或使酶活力下降,因此寻找耐有机溶剂的脂肪酶,使其在有机溶剂或含有机溶剂的环境中具有较高的催化活性,已成为脂肪酶研究领域的一个重要方向。
make (alcohol) unfit for drinking without impairing usefulness for other purposes
modify (as a native protein) especially by heat, acid, alkali, or ultraviolet radiation so that all of the original properties are removed or diminished
add nonfissionable material to (fissionable material) so as to make unsuitable for use in an atomic bomb