Methods: The PCR technique in which the primers were used as template each other was adopted to obtain the foreign DNAs for Cloning. 方法:以引物互为模板的聚合酶链反应合成目的基因片段。
The distribution of repetitive DNAs along chromosomes is one of the crucial elements for understanding the organization and the evolution of plant genomes. 重复DNA沿染色体的分布是认识植物基因组的组织和进化的要素之一。
Screening of highly virulent Beauveria bassiana isolates against Plutella xylostella in Zhangbei Screening and Identification of the Differential Genes of Genomic DNAs between Bovine Brucella Virulent and Vaccine Strain 对张北小菜蛾高毒力白僵菌菌株的筛选牛布鲁氏菌强毒株与疫苗株基因组差异基因筛选与鉴定
It is apparent that the polymorphism in chromosome DNAs exists in T.gondii isolates, although certain degree of homology is present in some strains. 结论弓形虫不同分离株既存在一定的同源性,但同时又显示染色体DNA多态性。
Satellite DNAs were first detected as distinct subfractions of the genome. 最初检测的随体DNA被当成为基因组的不同亚组分。
Screening and Identification of the Differential Genes of Genomic DNAs between Bovine Brucella Virulent and Vaccine Strain Immune protection of newcastle disease La Sota vaccine on different genotypes of newcastle disease isolates 牛布鲁氏菌强毒株与疫苗株基因组差异基因筛选与鉴定新城疫LaSota疫苗对不同基因型流行株的免疫保护
Diversity of Dioscorea zingiberensis from morphological characters to DNAs was discussed in this paper: ( 1) morphological and structure diversity;( 2) growth status; 就盾叶薯蓣的资源多样性主要特点作概要综述:(1)形态结构多样性;
Plasmid DNAs of constructs were separately used to immunize mice. 用重组质粒DNA肌肉免疫小鼠。
In this study Chinese autumn soybean were used as materials, and their genomic DNAs were analyzed with SSR markers. 选择中国秋大豆为试验材料,对基因组DNA进行SSR标记筛选和鉴定。
Method A pair of oligonucleotide sequences, which was based on the conserved region of 18s rRNA shared by medically important opportunistic fungi, was used as the primers to amplify the DNAs from medically important opportunistic fungi in a PCR assay. 方法以源于医学机会致病性真菌18SRRNA保守区的一对寡核苷酸序列为引物,利用聚合酶链反应对医学上重要机会致病性真菌DNA进行扩增。
Study on Ha-ras RFLP in gastric carcinoma and normal tissue DNAs of Chinese individuals 中国人胃癌组织和正常组织基因组DNA中癌基因Ha&ras的RFLP研究
A comparative study on the IFN gene construction, distribution, and copy number in two different human cell ( HF and Mern) chromosomal DNAs was carried out by using Southern technique. 本文采用Southern技术比较研究了α和β干扰素基因在干扰素诱生性高低不同的两种人细胞(HF和Mern细胞)染色体DNA中的组成情况。
The genomic DNAs prepared by both CTAB and the modified SDS were suitable for digestion with restriction enzyme. CTAB法和SDS改进法所得DNA都可直接应用于限制性酶切分析;
DNA, respectively. Comparative Study of Base Composition of DNAs DNA碱基组成的比较研究DNA碱基组成和碱基关联的进化
There were one or more plasmid-like DNAs in several plants, which might be not the key factors of CMS, but their existence expanded mtDNA's polymorphism. 在一些植物中还发现了线粒体质粒DNA,它们的存在增加了mtDNA的多态性。
The human papillomavirus ( HPV) 16 DNAs were prepared and purified from HPV-PBR_ ( 322) recombinant plasmid. 本文以人乳头状瘤病毒(HPV)16型-PBR(322)重组质粒,对大肠杆菌HB101进行转化实验。
At the same time, the rice DNAs yielded from yellow seedlings and green seedlings were compared, and the differences between HB method and NIB method in high molecular weight ( HMW) DNA isolation were also studied. 同时,比较了水稻绿苗和黄化苗的DNA得率,以及HB法和NIB法制备大分子质量(HMW)DNA的异同。
The genomic DNAs were extracted from tufted deer and were digested by Hind III respectively. 提取毛冠鹿高分子量基因组DNA,HindⅢ酶切毛冠鹿基因组DNA,将酶切后的基因组DNA转移至尼龙膜。
The size of the transgenes and the quality of the DNAs might affect the clone frequency. 不同基因的大小和质量会影响克隆的频率。
Plasmid-like DNAs have been discovered in green algae, red algae and diatom species etc. 已在绿藻、红藻与硅藻等真核藻中发现有质粒状DNA,其结构一般是环状或线状的双链DNA小分子。
Ten soybean cultivars 'total DNAs were extracted by CTAB method and used for identification and cluster analysis based on RAPD method. 用CTAB法提取了10个大豆品种总DNA,使用RAPD技术对其进行了品种鉴定和聚类分析。
Three methods were adopted to isolate genomic DNAs of stone fruit trees, such as apricot and peach. 采用3种方法分离杏、桃等核果类树种的基因组DNA,比较了其分离效果。
A simple and rapid PCR method were established in analyzing DNAs of seeds. 建立了适于种子DNA分析的快速简便的PCR方法。
Results showed that treatment with formaldehyde could increase the proportion of fetal DNAs in total plasma DNA. 结果发现采用甲醛处理的实验组提取获得的胎儿DNA占血浆总DNA的比例有了较大的提高。
Genomic DNAs from all the samples were amplified with cytoplasm universal primers of 6 chloroplast and 4 mitochondrial. 选用6个叶绿体和4个线粒体植物细胞质通用引物,以所有材料基因组DNA进行筛选。
In the present research, using CTAB extraction procedure, we isolated DNAs from tobacco and cabbage. 本实验采用CTAB法提取了烟草和甘蓝基因组DNA。
Four exons ( 1,4,7,9) of MTHFR were amplified by PCR, templated by the genomic DNAs. 以抽提的基因组DNA为模版,PCR扩增MTHFR基因的4个外显子(1,4,7,9)。