Methods The EtOH extract of radixes of Daphne genkwa was initially separated with silica gel chromatography eluted by petrol/ acetone gradient. 方法芫花根醇提物以硅胶柱层析,石油醚/丙酮梯度洗脱。
For each pair of amino acids, determine which will be eluted first from the ion-exchange column using a pH7.0 buffer. 确定下列每对氨基酸在pH7.0的缓冲液中,哪个先从离子交换柱上洗脱下来。
In early purifications of this type, antibody was eluted with dilute acid or base. 早期纯化这类抗体是用稀酸或稀碱洗脱。
The compositions of grease were eluted with petroleum ether, benzene, chloroform, ethyl acetate, acetone, ethanol and characterized by means of IR and NMR spectroscopy. 油脂部分依次以石油醚、苯、氯仿、乙酸乙酯、丙酮、乙醇为淋洗剂进行洗脱,利用红外光谱、核磁共振光谱法对所分离组分进行了结构鉴定。
Under optimum conditions, lead and cadmium can be enriched and eluted at the same time in the same water sample. 经过最佳条件选择,在同一水样中可以同时进行富集、洗脱铅和镉。
The adsorbed germanium can be quantitatively eluted with NH_ ( 4) F solution. 可有效地从含锗溶液中分离富集锗,被吸附的锗可用NH4F溶液定量洗脱。
Conclusion The total astragalosides are adsorbed by AB-8 resin collumn, eluted with water, 0.1% NaOH, then 20% ethanol in order, at last both the total astragalosides yield and purity have desirable higher level in 80% ethanol elution. 结论黄芪提取物上AB-8树脂柱,以水和0.1%NaOH洗脱后以80%乙醇洗脱黄芪总皂苷部位可使黄芪总皂苷的收率和质量分数达到较满意效果。
Resin loading gallium is eluted with basic complex eluant. 树脂吸附镓饱和以后,用特制的碱性络合淋洗剂淋洗。
At last, the isoflavone is eluted with gradient aqueous alcohol. 最后,利用梯度洗脱方法将大豆异黄酮由树脂柱上洗脱下来。
Phosphoric ions adsorbed by the zeolites can be eluted with some solutions. 沸石所吸附的磷可被某些溶液解吸下来。
When ⅰ < 6.000, SOD can not be eluted out from DEAE-cellulose column. pH7.4的磷酸盐缓冲液的离子强度低于6.000时,SOD不能从DEAE-纤维素柱上洗脱下来。
We determined remained pesticide in leek with SPE Column, eluted with solvent, and analysed with GC/ MS. 本文使用SPE柱萃取韭菜中的残留农药,经溶剂冼脱,利用色质联用仪(GC/MS)进行定性及定量分析。
In order to wipe of the fake positive, the eluted phages were absorbed by the negative rabbit sera. 在每一轮的筛选过程中,我们将洗脱下来的噬菌体再用正常的兔血清IgG进行吸附,去除了假阳性的噬菌体克隆,保证了阳性噬菌体克隆的特异性。
Based on the presence of high concentrations of the chaotropic agent guanidinium thiocyanate ( GuSCN), the nucleic acid could bind to silica particles ( SiO_2) and subsequently was eluted in a aqueous low-salt buffer. 在高浓度离液剂异硫氰酸胍(GuSCN)存在的条件下,核酸具有与硅珠(SiO2)颗粒结合、并能在较高温度下被低盐浓度溶液洗脱的特性。
For sequence analysis, fragments of 100~ 200 nucleotides in length were eluted from the gel. 从制备胶上洗脱长度在100~200个核苷酸的片段供顺序分析。
A GDX-101 column was used to capture the fragrance which was then eluted by ether for GC-MS analysis. 用GDX&101柱富集白兰瓜香气,用乙醚洗脱出,然后进行毛细管色谱-质谱分析。
The elution effect of acetone and petroleum ether as extractive solvent was similar, but the activity of recorded lipase polymers eluted with petroleum ether was higher. 选用丙酮和石油醚作为抽提溶剂,两者的洗脱效果相差不大,但是用石油醚洗脱后的聚合物内酶活性较高。
Three fractions were obtained by partial hydrolysis of powdery chitin in concentrated hydrochloric acid, followed by chromatography on charcoal column eluted with different concentrations of alcohol. 甲壳素细粉通过浓盐酸部分水解,活性炭吸附层析,以不同浓度的乙醇洗脱得三个组分。
The pyrethroid pesticides were separated by Hypersil ODS column and eluted using acetonitrile/ water with a gradient program. 采用HypersilODS色谱柱,以乙腈/水为流动相、梯度洗脱进行分离。
The SEM results demonstrated that the sporoderm was ground and the inclusion of the spores eluted in groups. 扫描电镜结果表明,灵芝孢子经过高速剪切粉碎破壁后,灵芝孢壁被打碎,孢子内含物质呈团状溶出。
Some protein is adsorbed and eluted in the control groups ( no metal ions and no IDA or metal ions), even more than the one using Co2+-IMAN. 对照组(无金属离子、无IDA和金属离子)也有少量目标蛋白被吸附和洗脱,甚至好于Co2+-IMAN。
Proteins were eluted directly in SDS sample buffer for Western blot analysis. 8. 使用SDS样本试剂直接洗脱蛋白质,然后进行蛋白印迹分析。
The separation conditions of astaxanthin isomer were optimized by the selection of the chromatography column, eluted methods, mobile phase, mobile phase ratio and the column temperature. 分别从色谱柱,洗脱方式与流动相的选取,流动相配比与流速的确定,柱温的选择等几个方面对虾青素异构体分离的色谱条件进行了优化。