Study on Over-expression of Bacillus Subtilis Neutral Endoglucanase Gene in Pichia Pastories and Its Structural Domain Reconstruction 内切葡聚糖酶基因在毕赤酵母中高效表达及其结构域重构研究
Saturation mutagenesis of three amino acid positions consisting of the active site of an endoglucanase from termite Coptotermes formosanus Over-expression of Endoglucanase Gene in Pichia pastoris 台湾家白蚁内切葡聚糖酶活性中心氨基酸的饱和突变内切葡聚糖酶基因在毕赤酵母中高效表达研究
Cloning, sequencing, and expression of endoglucanase gene from Bacillus subtilis C-36 in Escherichia coil 枯草芽孢杆菌C-36内切葡聚糖酶基因的克隆及其在大肠杆菌中融合表达
Cloning and Sequencing of Endoglucanase Gene from Bacillus megaterium 巨大芽孢杆菌内切葡聚糖酶编码基因的克隆及序列分析
The results show that the deinking rate of the waste paper pretreated with NaOH was increased by 10% than that with endoglucanase EGI deinking. 结果表明,与内切纤维素酶EGI脱墨相比,经NaOH预处理后再酶处理可提高油墨脱出率约10%。
This article dealt with cloning and sequencing of chitinase and endoglucanase genes of Bacillus spp. 本研究旨在从两株生防菌中分别克隆几丁质酶和内切葡聚糖酶的编码基因,并进行测序。
CDH had little synergism with cellobiohydrolase I ( CBH I) or endoglucanase I ( EG I) in cotton cellulose degradation. CDH与纤维二糖水解酶或内切纤维素酶在降解棉花纤维素时没有表现出协同作用。
Cellulose is degraded mainly in nature by the synergistic action of endoglucanase ( EG) and cellobiohydrolase ( CBH). 天然纤维素的有效酶解取决于外切葡聚糖纤维二糖水解酶(CBH)和内切葡聚糖水解酶(EG)的协同作用。
Molecular Cloning and Expression of An Endoglucanase Gene from Bacillus subtilis in Escherichia coli 枯草杆菌内切葡聚糖酶基因的克隆及表达
Construction Genomic Library of Extremely Thermophilic Anaerobic Bacterium BANG 2 and Cloning of Endoglucanase Gene Fragments 极端嗜热厌氧纤维素分解菌基因文库的构建及其内切葡聚糖酶基因片段的克隆
Many results showed that endoglucanase ( EG) played key role on modification of plant fibre. 大量研究结果表明,内切型纤维素酶在植物纤维酶法改性中起着关键作用。
β-1,4-endoglucanase, also called Cx enzyme or CMC enzyme, belongs to the cellulases. It can degrade the macromolecule cellulose into cellobiose, and very important in the process of the hydrolysis of celluloses by cellulases. 内切型B-1,4-葡聚糖苷酶(也称Cx酶或CMC酶)属于纤维素酶系,能将大分子纤维素降解为纤维二糖,在纤维素酶系水解纤维素的过程中起关键作用。
Penicillium sp. P6 possessing strong degrading ability of lignite could produce stronger activity of peroxidase ( including lignin peroxidase and manganese peroxidase), under the condition of liquid cultivation and possess esterase activity; but could not produce endoglucanase and xylanase. 具有强降解褐煤能力的青霉菌株P6在液体培养条件下,可产生较强的过氧化物酶(包括木素过氧化物酶和锰过氧化物酶)活性,并具有酯酶活性,不产生内葡聚糖酶和木聚糖酶。
The results showed that the endoglucanase gene has been cloned. 结果表明已克隆到邦2菌纤维素酶系中的内切葡聚糖酶基因(ED基因)片段。
The Protection of Tween 80 for Endoglucanase Against the Inhibition of Ethanol Tween80解除乙醇对内切葡聚糖酶(Cx)的抑制作用
Genome library methodology was adopted for cloning of endoglucanase encoding genes. 内切葡聚糖酶基因的克隆采取基因文库方法。
Used this as a template, the endoglucanase cellulose was cloned through PCR reaction. 2. 以此为模板,通过克隆测序得到纤维素葡聚糖内切酶。
The results are as follows: The endoglucanase target gene has high homology within the glucan produced by other bacterial enzymes. 结果如下:目的基因与其他细菌所产的葡聚糖内切酶同源性很高。
Endoglucanase can be widely applied to industrial production of producing fuel ethanol and pulp treatment, and has a good application prospect. 内切葡聚糖酶的活力大小直接影响纤维素的降解效率,其在工业生产生物乙醇和纸浆处理中应用广泛,具有良好的应用前景。