A total of22 restriction endonucleases were used to investigate the mitochondrial DNA polymorphism of Sanjiang White pigs. 采用22种限制性内切酶分析了三江白猪线粒体DNA的多态性。
Isolation and Purification of Restriction Endonucleases Bgl I and Pst I 限制性内切酶BglⅠ与PstⅠ的提取和纯化
Quantitative detection of methylation status in the multi-drug resistance 1 gene by competitive polymerase chain reaction combined with restrictive endonucleases 限制性内切酶结合竞争聚合酶链反应定量检测在多药耐药基因甲基化状态中的应用
The splicing system is a language generative mechanism based on splicing operations, where splicing operations are the mathematical abstractions of DNA strands recombinant behaviors under restriction exonucleases, endonucleases, DNA ligases and DNA polymerases. 剪接系统是将剪接运算当作基本算子的一种语言生成器,其中剪接运算是对在限制性内切酶、DNA连接酶、DNA聚合酶和外切酶作用下,DNA链进行重组过程的数学抽象。
Rejoining fidelity of DNA double-strand breaks induced by restriction endonucleases in CHO cells 限制性内切酶诱发DNA双链断裂在细胞中的修复忠实性
Then the deletions were mapped by restriction endonucleases analysis. Thus, the location of transposition genes of Tn233 ( CH) was verified. 对这些缺失变种在DNA上缺失的区域进行限制图分析,确定了转座子Tn233(CH)中转座基因的位置。
Analysis of Herpes Simplex Virus Genomes with Restriction Endonucleases 单纯疱疹病毒基因组的限制性核酸内切酶分析
In order to discuss the molecular biological character of Y. pestis in the northwest pastures in Jilin province, the sorts and Restriction DNA endonucleases map of Plasmids from 30 strains of Y. 为探讨吉林西北部草原鼠疫菌分子生物学性状,本研究测试了该地区30株鼠疫菌携带的质粒种类和质粒DNA酶切图谱。
With some restriction endonucleases digestion the hybridization pattern of rice DNA showed more than 40 bands with different intensity mainly consisting of the strong and weak hybridization bands. 某些限制性内切酶消化过的水稻DNA,其图谱呈现出多达40条以上的杂交带,包括强杂交带和弱杂交带两种类型。
Results Endonucleases digestion confirmed that I κ B α gene cDNA has been inserted into the prokaryotic expression vector pET-32a (+). 结果酶切鉴定证实人IκBα基因cDNA已插入原核表达载体pET32a(+)。
A map of 8 restriction endonucleases and structure of electron microscope of Chinese mitochondrial DNA 中国人线粒体DNA的8种限制酶图及其电镜结构
Hairpin Probes for Real-time Assay of Restriction Endonucleases 实时检测限制性内切酶活性的发夹型荧光探针
18 patterns and 14 haplotypes were observed with the digestion of 6 endonucleases. 6种限制性内切酶共获得18种酶谱和14种基因型。
The restriction endonucleases from Bacillus 芽孢杆菌中的限制性核酸内切酶
Artificial nucleases have high selectivity just as endonucleases, they can cleave DNA at any locations that the chemists have designed, and they can be used in molecular biological areas such as isolation genes, DNA location inducers and new chemical therapy etc. 人工核酸酶具有限制性核酸内切酶的高度专一性,能在人们预先设计的任何位点切割DNA,可应用于基因分离、DNA定位诱变、新的化学治疗等分子生物学领域。
On the ground of the information of RFLP marker we had, we synthesized the PCR primers, devised restriction endonucleases, and transferred RFLP with complex operation and high cost into CAPS marker, whose operation is simple and applicable with low cost. 根据已有的RFLP标记信息,合成PCR引物、设计限制性内切酶,就可把操作复杂、成本较高的RFLP标记转化为操作简单实用,成本适宜的CAPS标记。
The sites of restriction endonucleases were analysed and restriction site map of the cloned fragment was drawn. 对克隆片段进行了酶切位点分析,并做了限制性内切酶图谱。
Restriction endonucleases fingerprinting-single strand conformation polymorphism: an efficient method to screen mutations in long segments 限制酶切指纹-单链构象多态性&一种有效的大片段中突变的检测方法
Sister Chromatid Exchanges Induced by Restriction Endonucleases 限制性内切酶诱发的姊妹染色单体互换
Adopting normal DNA recombination protocols: digestion with restriction endonucleases and ligation with T4-DNA ligase, the two genes were cloned into expression plamid pET-28a of Escherichia coli. 采用常规的基因重组技术,即限制性内切酶酶切和T4-DNA连接酶连接,将这两个基因克隆到大肠杆菌表达质粒pET28a中。
Then the antibody genes and the phage display vector were digested by restriction endonucleases Sfi ⅰ and Not ⅰ. 然后利用限制性内切酶SfiⅠ、NotⅠ分别双酶切抗体基因和噬菌体展示载体。
Analysis of the Chromosome Banding with Restriction Endonucleases 用限制性内切酶诱导染色体显带的分析
The activities of restriction endonucleases were found in10 out of66 strains of Bacillus isolated and examined in our country. 我们从66株国内收集的芽孢杆菌中筛选出10株菌株,它们含有限制性核酸内切酶活力。
In the absence of endonucleases, the fluorescence of QDs is quenched through energy transfer effect, and the nanoprobe stays in the "OFF" state. 在没有核酸内切酶存在时,QDs的荧光通过能量转移被猝灭,纳米探针处于关闭状态。
Ribonuclease A superfamily is a class of endonucleases which can hydrolyze the RNA nuclear acid. Their homologous genes are clustered on the chromatin. 核糖核酸酶A超家族是一类可以水解RNA的核酸内切酶,其同源基因通常在染色质上成簇排列。
Using the traditional methods of molecular biology, every time when we need to replace the vector for another, we should experience the whole cloning process, including PCR, endonucleases digestion, connectivity, transformation, identification and DNA sequencing analysis. 使用传统的分子生物学方法,每更换一次载体,都要从头构建,需经历PCR扩增,酶切,连接,转化,鉴定和DNA测序的过程。