Construction and Identification of the Recombinant Eucaryotic Plasmid Encoding Survivin Gene of Mice 小鼠survivin基因重组真核表达载体的构建及鉴定
CREB as a transcriptional regulation factor in eucaryotic cell carries out the function by itself phosphorylation. CREB是一种细胞核内调控因子,它通过自身磷酸化实现调节转录的功能。
In addition, the EO gene of hog cholera lapinized vaccine ( HCLV) strain was expressed in the prokaryotic and eucaryotic systems, and the recombinant proteins were preliminarily analyzed by immunological method. 同时将兔化弱毒株EO基因分别置于原核与真核表达系中进行了表达,并对重组蛋白进行了初步的免疫学分析。
The study of interaction of Hb with the erythrocyte membrane could provide information on the interaction of cytoplasmic protein with biomembrane in the eucaryotic cells. 高铁血红蛋白及红细胞膜去胆固醇皆能修饰血红蛋白和膜的相互作用。
Construction of T-cell Receptor γ V_1 Rearrangement Gene Expression Vector in Eucaryotic Cells T细胞淋巴瘤TCRγV1重排基因真核表达载体的构建
Microsatellite is the simple serial repetitive DNA sequence widely distributed in the genome of prokaryocytes and eucaryotic cells with high degree of polymorphism and very low variation rate, showing the Mendel's codominant inheritance. 微卫星是广泛存在于原核及真核细胞基因组中的简单串联重复DNA序列,并具有高度多态性,变异率极低,以孟德尔共显性方式遗传。
The eucaryotic NP-1 expression vector was transformed into the NR deficient mutant of C. 将构建好的兔防御素真核表达载体电击转化椭圆小球藻硝酸还原酶功能缺失突变体。
AIM To clone cDNA of N terminal fragment of human bactericidal/ permeability increasing protein ( BPI), to construct the procaryotic and eucaryotic expression vectors and to express the cDNA in E. coli and CHO cells respectively. 目的克隆杀菌/通透性增强蛋白(BPI)N端cDNA,构建原核和真核表达载体,分别在大肠杆菌和CHO细胞中表达BPI蛋白。
Identifying exons in an eucaryotic gene by statistical method 应用统计方法确定真核基因外显子
The baculovirus-insects expression system is one of the promising eucaryotic systems in which a lot of foreign genes of economic value have been efficiently expressed. 同时杆状病毒-昆虫(虫体)表达系统是最近发展起来的高效表达外源基因的真核表达系统。
A neutrophile peptide eucaryotic expression vector was constructed. 构建了兔防御素蛋白的真核表达载体。
Study on protein expression and antigenicity detection of hepatitis C Virus envelope protein E2 in prokaryotic and eucaryotic expression systems. The gene that encoding. 为研究丙型肝炎病毒(HCV)包膜蛋白E2在原核细胞和真核细胞中的表达,并对表达蛋白的抗原性进行检测。
Conclusion Retroviral expression vector with HBV/ C gene can transfect into eucaryotic cells effectively and express the goal gene steadily. The recombinant cells may be used in the systematic studies on the biological significance of HBV/ C gene variant. 结论HBV/C基因逆转录病毒表达载体有较高的转染效率,目的基因能在宿主细胞中稳定表达,有利于系列研究HBV/C基因变异的生物学意义。
Significance of eucaryotic organisms in the microfossil flora of Changcheng System 论长城系微化石群的真核生物意义
These results suggested that T7 promoter functions as cis-acting elements of RNA polymerase ⅱ transcriptional system in eucaryotic cells. 实验结果表明T7启动子可作为RNA聚合酶Ⅱ的顺式作用因子。
Preliminary objective of this study was to transfer R-M genes into mammalian cells and observe whether they could resist eucaryotic viruses as in procaryotic cells. 本课题的原设想是将R&M基因导入哺乳动物细胞,模拟其在细菌中的天然防御机制,进行抗真核病毒的研究。
Cloning and Sequencing of Matrix Metalloproteinase-2 C End Segment Pex and Construction of its Eucaryotic Expression Vector 基质金属蛋白酶-2C端片段pex的克隆、测序及真核表达载体的构建
Eucaryotic Expression and Biological Activities of Rubella Virus Glycoprotein E1 风疹病毒包膜糖蛋白E1的真核表达及其生物学活性研究
Methods We construct the rat antisense TIMP 1 recombinant plasmid which can be expressed in eucaryotic cells by RT Nest PCR and the technique of recombinant DNA. 方法运用逆转录巢式(RTPCR)和重组DNA技术,构建大鼠反义TIMP1真核细胞表达质粒,经脂质体包埋后,利用腹腔注射将其导入免疫性大鼠肝纤维化模型体内;
F-actin is polymerized by G-actin which participates the formation of the netlike protein system of the complex skeleton in the eucaryotic cell. 微丝(F-actin)是由肌动蛋白单体(G-actin)聚合形成的,它参与了真核细胞中复杂的细胞骨架网状蛋白体系的形成。
With flank sequence of HA gene of vaccinia virus, four eucaryotic expression plasmids containing cDNA of rabies virus glycoprotein gene under the control of different hybrid promoters of pox virus were constructed and transfected into BHK 21 cells infected with wild type vaccinia virus. 以血凝素(HA)基因为侧翼,将狂犬病病毒糖蛋白基因cDNA置于不同类型的痘苗病毒复合启动子下游,构建了4种表达质粒。
Fine structure observation of chromatin in eucaryotic cells 真核细胞染色质结构的观察
Objective This study was designed to construct the eucaryotic expression vector of TCR γ V_1 rearrangement gene. 目的构建含TCRγV1重排基因的真核表达质粒。
Construction of eucaryotic expression plasmid carrying the recombined human BMP-7 gene and its expression in chondrocytes 重组人BMP-7真核表达质粒的构建及转染软骨细胞后的表达
Ve To study the expression of murine interleukin-18 ( mIL-18) in eucaryotic cells transfected with retroviral vector encoding mIL-18 cDNA, which is the first step towards mIL-18 gene therapy of cancer. 癌症的基因治疗目的构建小鼠白细胞介素-18(mIL-18)逆转录病毒载体,实现mIL-18在小鼠H22肝癌细胞中表达。
Cytochrome oxidase is the terminat oxidase of the respiratory chain in eucaryotic cell. 细胞色素氧化酶是真核生物细胞呼吸链终末氧化酶,是生物体内能量生成的重要步骤。
Application of the Single Cell Gel Electrophoresis in Eucaryotic Microorganisms 单细胞凝胶电泳在真核微生物上的应用
Mitochondrion which possesses its own genome is a fundamental and important organelle that exists in eucaryotic organism. Mitochondrion also participates in a lot of metabolic process which relates with apoptosis, disease and senility. 线粒体作为绝大多数真核细胞生物体内的一种重要且基本的细胞器,拥有自己的基因组参与疾病、衰老和细胞凋亡等相关代谢过程。