Species-specific secondary antibodies labeled with horseradish peroxidase ( HRP) and alkaline phosphatase ( AP), and spectrally distinct fluorogenic substrates for each enzyme, are used for detection. 用带有不同荧光底物的辣根过氧物酶和碱性磷酸酶标记的种属特异的二抗来检测。
Methods and Results& The NIRF imaging agent consists of the CatK peptide substrate GHPGGPQGKC-NH2 linked to an activatable fluorogenic polymer. 方法及结果:短距红外线荧光显影剂含有组织蛋白酶K缩氨酸感光底物GHPGGPQGKC-NH2,其连结在一个可激活的荧光底物激活体上。
Fluorogenic quantitative PCR method for Plasmodium vivax 间日疟原虫荧光定量聚合酶链反应检测方法的建立
Both molecules evoked intrasynaptosomal generation of reactive oxygen species measured by fluorogenic probe, 2'7 '-dichlorofluorescin diacetate. 通过2'7'-二氯双乙酸盐荧光探针测量,两种分子都诱发突触内产生活性的氧物质。
In order to meet the analysis requirement, this article introduced the main technological method and concrete application of the independently developed isotope X fluorogenic analysis instrument. 为满足分析要求,介绍了自行研制的同位素X射线荧光分析仪的主要技术方法及具体应用。
Detection of respiratory syncytial virus in children with respiratory tract infections by nucleic acid amplification fluorogenic quantitative assay 呼吸道感染患儿呼吸道合胞病毒核酸扩增荧光定量检测及分析
The thesis focuses on gene diagnosis of diseases, and six new homogenous fluorogenic probes are developed. 绪论部分对现有的几种均相荧光基因探针技术进行了评述,指出了它们所存在的优势与不足。
Qualification of hyperpolarization-activated cyclic nucleotide-gated cation channel mRNA using the fluorogenic quantitative RT-PCR method 采用荧光定量RT-PCR法检测超极化激活环核苷酸门控阳离子通道
Conclusion The fluorogenic Quantitative PCR method for the detection of Plasmodium falciparum is simple and accurate. 我们在常规PCR技术的基础上,建立了检测恶性疟原虫的荧光定量PCR方法。
Objective To establish fluorogenic quantitative PCR method for detecting the gene expression of transcription factors. 目的建立荧光定量聚合酶链反应(fluorogenicquantitativePCR)检测方法,以便快速、准确地定量检测细胞株转录因子表达强度。
Detection of HPV DNA in patents with condyloma acuminatum using fluorogenic quantitative PCR technique 用核酸荧光定量PCR技术检测尖锐湿疣HPV的研究
Results The expression of TNF-α mRNA in PBMC for tumor patients was more high than that of normal persons; The semi-quantitative RT-PCR results also demonstrated that TNF-α level varied as detected with real-time fluorogenic quantitative RT-PCR, but less sensitive and accurate. 结果实时荧光定量RT-PCR检测肿瘤患者外周血单个核细胞TNF-αmRNA表达明显高于正常人,且半定量RT-PCR结果显示TNF-α出现类似的变化趋势。
Application of real-time fluorogenic PCR assay for the detection of Campylobacter jejuni 应用实时荧光PCR技术检测空肠弯曲菌
Purpose To establish a FQ? PCR ( fluorogenic quantitative RT? PCR) method for detecting the expression of tissue factor gene in tissue and investigate the relationship between TF mRNA expression and tumor. 目的建立检测组织因子(TissueFactor,TF)mRNA荧光定量的方法,了解组织中TF的表达水平,研究TfmRNA的表达与肿瘤的关系。
Methods One siRNA-MRP was designed, synthesized and transfected into breast cancer cell line MCF-7/ adr. Expression level of MRP mRNA was determined with real-time fluorogenic quantitative PCR. 方法设计针对MRP基因的siRNA,转染乳腺癌MCF-7/adr细胞,用实时荧光定量PCR分析mrpmRNA的表达;
Methods: Herpes virus DNA, cytomegalo virus DNA, coxsackie virus RNA were detected by the Fluorogenic Quantitative PCR technology in 41 children with TORCH infection and EEG was done in those patients with positive results. 方法:使用荧光聚合酶链反应技术检测41例病儿单纯疱疹病毒DNA,巨细胞病毒DNA,柯萨奇病毒RNA,对其中为阳性的病儿进行脑电图检查,比较检查结果。
Objective To develop a fluorogenic probe PCR assay for rapid detection of salmonella species. 目的建立一种快速检测沙门菌的荧光探针杂交聚合酶链反应(PCR)方法。
Use of a fluorogenic probe in a polymerase chain reaction assay for rapid detection of salmonella species 荧光探针杂交-聚合酶链反应检测沙门菌的研究及应用
Synthesis and Study of Fluorogenic Property Four Kinds of Coumarin Sulphonyl Chloride Derivatives 四个香豆素磺酰氯衍生物的合成及其荧光性能的研究
Studies on the Reaction Mechanism and Properties of a New Peroxidase Fluorogenic Substrate Dihydro-2 ( 1H)-quinoxalone 过氧化物酶新型荧光底物3,4-二氢-2(1H)-喹喔啉酮的反应机理及性能的研究
Double antibody enzyme-linked immunosorbent assay and fluorogenic quantitative polymerase chain reaction were used to detect the level of sIL-2R and HBV-DNA respectively. sIL2R采用双抗体夹心间接酶联免疫吸附测定法,HBVdna采用荧光定量聚合酶链法,丙氨酸转氨酶(ALT)采用赖氏法。
The detection of mdr-1 gene expression using fluorogenic probe quantitative RT-PCR method 荧光定量RT-PCR检测mdr-1基因表达
Methods: Expression of bcr/ abl 'gene was measured with fluorogenic probe quantitative RT-PCR ( FQ-RT-PCR) in 34 cases of leukemia at different disease-developing stage. 【方法】用荧光定量RT-PCR法(FQ-RT-PCR)测定34例不同类型白血病及处于不同疾病阶段的白血病病bcr/abl基因的表达。
Objective To search the better molecular biology method in the detection of HCMV by comparing the role of common PCR with Fluorogenic Quantitative PCR ( FQ-PCR). 目的比较常规PCR法与荧光定量PCR(FQ-PCR)法在人巨细胞病毒(HCMV)检测中的作用,寻找更适合于HCMV检测的方法。
Objective To establish fluorogenic quantitative PCR method for detecting Plasmodium falciparum. 目的建立恶性疟原虫荧光定量聚合酶链反应(PCR)检测方法,为快速、准确地定量检测恶性疟原虫奠定基础。
Method 363 blood donors were detected by Fluorogenic quantitative PCR. 方法采用FQ-PCR法定量检测363例献血员的血清标本。
Based on this mechanism, a fluorogenic method for the determination of S2-was developed. 据此,建立了一种基于PET机理的测定硫离子的荧光探针。
Based on the above mechanism, a fluorogenic method for the determination of hypochlorite ion was developed. 基于以上原理,建立了一种测定ClO-的新型荧光分析法。