Real time ex vivo detection and dynamic monitoring of in vivo expression of secreted luciferase gene injected by hydrodynamic method 体外实时动态监测水动力注射分泌型荧光素酶基因的表达
ObjectiveTo construct glypican-3 ( GPC3) promoter luciferase reporter gene vector, and to analyze its transcriptional activity. 目的构建磷脂酰肌醇蛋白聚糖3(GPC3)启动子荧光素酶报告基因载体,并验证其转录活性。
The human GRP94 promoter was then cloned upstream of the luciferase gene and showed enhanced activity in hypoxic conditions. 随后人GRP94启动子克隆了荧光素酶基因上游区且缺氧环境中活性增强。
Comparison of TTR and CMV Promoters in vivo and in vitro via a Secreted Luciferase Reporter System 用分泌型萤光素酶报告系统比较TTR启动子与CMV启动子的体内外表达特性
Results A series of truncated NOS1 promoter luciferase reporter vectors were constructed and identified. 结果构建并鉴定了系列截短的NOS1启动子萤光素酶报告基因载体;
The researchers developed a new screening system, based on a naturally fluorescent protein called luciferase, to identify small molecules that inhibit proteins that turn genes on and off. 在天然荧光蛋白荧光素酶的基础上,研究者发展了一种新的筛选系统,此系统可以用来鉴定抑制启动和关闭基因蛋白的小分子。
Construction and identification of human Puma promotor luciferase report gene vector 人Puma启动子荧光素酶报告基因的构建和鉴定
Construction of luciferase reporter gene plasmid containing hBD-2 and mutant correlated with Crohn's disease β-防御素-2荧光素酶报告基因载体及克罗恩突变体的构建
Construction of Recombinant Retroviral Vector Containing Firefly Luciferase Reporter Gene 萤火虫荧光素酶报告基因重组逆转录病毒载体的构建及鉴定
Viviperception of Transgenic Human Embryonic Stem Cells Labeled by Luciferase 荧光素酶标记转基因人胚胎干细胞的活体观察
Construction of a luciferase reporter vector with resistance of neomycin 带新霉素抗性的萤光素酶报告载体的构建
Objective To study the DTA vector containing the Ewing family of tumors EWS-FLI1 binding sequence depresses the co-transferred luciferase reporter plasmid. 目的研究含尤文家族肿瘤EWSFLI1结合序列的DTA载体对报告基因表达的抑制作用。
Quantitative detection of NADH by in vitro bacterial luciferase bioluminescent 细菌荧光素酶体外发光体系的建立及应用于NADH定量检测
The transcriptional activation activity of GR was evaluated through method of relative activity of luciferase. 相对荧光素酶活性法检测GR转录激活功能的变化。
Transcriptional activity of NF-κ B was determined using luciferase reporter gene assay. 应用荧光素酶报告基因技术检测DHMEQ对NF-κB基因活性的影响;
Conclusion The recombinant plasmid highly expressing luciferase has been successfully constructed in Drosophila S2 cells. 结论:成功构建了果蝇S2细胞中高表达荧光素酶的重组质粒pGL3-pac。
Methods: Cell culture, transient transfection and luciferase assay. 方法:细胞培养,瞬时转染和报告基因检测。
Third, we use Luciferase reporter gene to screen phytoestrogen from Herba Epimedii and Wolfberry fruit. 第三,用荧光素酶报告基因,观察淫羊藿和枸杞的雌激素作用。
We addressed this question with the method of luciferase reporter gene. 我们用荧光素酶报告基因的方法,探讨了这个问题。
Methods The method of luciferase reporter gene was used. 方法采用荧光素酶报告基因方法。
The species, structure and applications of luciferase were researched. 文章就荧光素酶的种类、结构及应用进行了研究。
Secondly, we used the dual luciferase reporter gene assay system to confirm the target gene. 其次,采用双荧光素酶报告基因检测系统进行靶基因的确认。
We generated a luciferase reporter construct containing the Wnt signaling pathway transcription factor binding sites. 我们构建了包含FLOT2启动子和Wnt信号通路转录因子结合位点的荧光素酶报告基因载体。
Enhanced green fluorescent protein ( GFP) plasmid and Luciferase plasmid was selected as the report genes, respectively. 选用了两种分别编码绿色荧光蛋白和虫荧光素酶的质粒。
AR expression and promoter activity were evaluated with RT-qPCR, western blot analysis and luciferase reporter assay. 此外,通过相应的细胞实验(实时定量PCR、免疫印迹和荧光报告分析等实验)测试AR的表达和启动子功能。
To confirm this hypothesis, we used dual luciferase reporter gene assay. 为了支持这一观点,我们用双荧光素酶报告基因分析的方法进行验证。
The activity of luciferase was detected using dual luciferase reporter system. 通过双荧光素酶报告系统检测荧光素酶的活性。
We further studied the function of this fragment with luciferase report assay. 我们用luciferase报告基因的方法进一步研究了这个片段的功能。
The consequences of WB and Luciferase Reporter Assay showed that Nef down-regulated AR expression level and PSA promoter activity. WB和虫荧光素酶报告实验表明,Nef在体外可以特异性的下调前列腺癌细胞中的AR的表达水平以及PSA启动子的活性。