Purpose To express and purify the fused protein containing 20 residues of amino-terminal of influenza hemagglutinin HA2 and ten lysines, and investigate its ability of binding plasmid. 目的表达和纯化流感病毒血凝素HA2氨基末端20个氨基酸与10个赖氨酸的融合蛋白HA20-lys10,并观察其与质粒结合的能力。
With site directed mutagenesis technique,α 99 and β 82 lysines were mutated to cysteines. 利用寡核苷酸介导的定点突变技术将α99、β82位的Lys突变为Cys。
By the sequence analysis, excluding the cystine, the glycine in Loop region, the praline and isoleucine in the head and tail of α-helix and lysines in C-terminus were also shown high conservation in 62 defensins. 氨基酸序列分析发现,62条防御素序列中除保守的半胱氨酸外,Loop区的甘氨酸;α-螺旋结构首尾的脯氨酸和异亮氨酸;C末端的赖氨酸同样高度保守。