In this paper, we have developed several detection methods for small molecules-protein interaction and copy number variations based on nucleic acid amplification methods as follows: ( 1) We developed a RNA transcription nanomachine by assembling T7 RNA polymerase on a small molecule-labeled DNA heteroduplex. 本文发展了几种检测小分子-蛋白质相互作用以及拷贝数变异的核酸扩增检测方法:(1)结合T7聚合酶与一个小分子标记的DNA双链,发展了一种RNA转录器件。