NCCLS has organized a working group which is currently performing studies to evaluate the disk and MIC methods for S.maltophilia and B.cepacia. NCCLS已组织了一个工作组,目前正在进行嗜麦芽窄食单胞菌和洋葱伯克霍德菌的纸片法与MIC的评价研究。
Methods The bacterium determine that adopts API system and craft law, the quick test of medicine adopts K-B law, the result judge that carries out according to NCCLS standard. 方法细菌鉴定采用API系统和手工法,药敏试验采用K-B法,结果判定按NCCLS标准执行。
Objective To compare the Etest and microdilution methods according to the National Committee for Clinical Laboratory Standards ( NCCLS) for determining the minimum inhibitory concentration ( MIC) of fluconazole. 目的观察氟康唑对念珠菌的敏感性,并研究酵母菌的体外抗真菌药敏试验Etest法和美国国家临床试验标准化委员会(NCCLS)微量法的可比性。
Extended spectrum β-lactamases ( ESBLs) were tested according to NCCLS Test. NCCLS方法检测超广谱β-内酰胺酶(ESBLs);
METHODS: The sensitivity tests of 123 strains from clinical isolated bacteria to Ise and other 17 antibiotics were determined by standard ( NCCLS). 方法:按美国NCCLS药敏纸片扩散法检测123株临床分离菌对异帕米星和其他17种抗生素的敏感性。
Methods 78 clinical and environmental isolates of Cryptococcus neoformans was tested by a modified National Committee for Clinical Laboratory Standards ( NCCLS) microdilution assay, and the interactions were examined using a checkerboard design. 方法采用美国国家实验室标准委员会(NCCLS)M27A方案推荐的酵母菌微量稀释法及其微量稀释棋盘法,检测78株新生隐球菌临床和环境分离株对卡泊芬净与特比萘芬的体外抗菌活性及其联合抗菌活性。
In order to compare the difference of antibiotic resistant model between two pig farms, antimicrobial susceptivity test were carried out to Escherichia coli by using the Kirby-Bauer method recommended by National Committee for Clinical Laboratory Standards ( NCCLS). 按照美国临床实验室标准委员会(NCCLS)推荐的Kirby-Bauer药敏纸片法,对从2个猪扬分离到的大肠杆菌进行了17种抗菌药物的敏感性测试。
Methods K-B test was used for the antibiotics susceptibility test and the results were read based on National committee for Clinical Laboratory standards ( NCCLs). 方法:连续检测2002年至2004年本院铜绿假单胞菌的耐药状况。药敏试验采用K-B纸片法,判定标准依据美国国家临床实验室标准化委员会(NCCLS)标准。
Extended spectrum beta-lactamases ( ESBLs) of Escherichia coli and Klebsiella pneumoniae were detected by method recommended by NCCLS. 根据NCCLS推荐方法检测大肠埃希菌和肺炎克雷伯菌产超广谱β内酰胺酶(ESBLs)的情况。
Methods: Cefoxitin three-dimensional test and disk diffusion confirmation method recommended by NCCLS were used to detect AmpC enzyme and ESBLs respectively. 方法:采用头孢西丁三维试验检测AmpC酶;NCCLS推荐的纸片扩散确证法检测ESBLs;
METHODS The antimicrobial-resistant genes were analyzed by PCR. The inducible resistance of erythromycin to clindamycin were checked by D-test according to the standards of CLSI ( NCCLS). 44株阴沟肠杆菌耐药基因分析方法用PCR技术检测耐药基因,采用CLSI(NCCLS)推荐的D试验方法测定红霉素对克林霉素的诱导耐药表型。
Conclusion Double-disc synergy test and NCCLS confirm test are simple, effective methods which are suitable for the basic clinical microbiological laboratory to detect ESBLs, the latter is the standard method for evaluating other tests. 结论双纸片协同法、纸片确证试验简单、有效,适用于广大基层实验室开展和推广,而纸片确证试验应作为临床检测产ESBLs菌的标准方法;
K-B paper disk diffusion and broth quantitative dilution method in accordance to the NCCLS Standards were performed for drug susceptibility. 药敏试验应用K-B纸片扩散法和肉汤定量稀释法,按NCCLS规定的标准进行。
Methods A total of 110 clinical isolates of nonrepetitive drug-resistant K. pneumoniae and E. coli were detected by three-dimensional extract test for AmpC β-lactamases and phenotypic confirmatory test based on the National Committee for Clinical Laboratory Standard ( NCCLS) criteria for ESBLs. 方法110株临床分离无重复肺炎克雷伯菌与大肠埃希菌耐药株,采用酶提取物三维实验检测AmpC酶,美国临床实验室标准委员会(NCCLS)表型筛选和确认实验检测ESBLs;
Methods Pathogenic bacteria were separated from the sputum samples collected from the in_patients in the year of 1999. K-B method was used and drug resistance was identified according to the NCCLS criteria. 方法收集1999年住院病例痰液标本,分离致病菌,采用KB法进行药敏试验,根据NCCLS标准判断细菌耐药性。
Methods The bacteria were characterized by ATB expression automatic analytic system, their drug-resistances were analysed with drug sensitivity detector; the suspiciously produced ESBLs was detected by standard screen test ( recommended by NCCLS). 方法使用ATBExpression全自动鉴定及药敏测试仪对细菌进行鉴定及耐药性分析,ESBLs的检测按NCCLS推荐的初筛、表型确认试验法操作。
Using paper diffusion and Mic method of Microscan system to make drug sensitive test, judging the data obtained according to the National Committee for Clinical Laboratory Standards 1999 ( NCCLS 1999). 采用纸片扩散法、Microscan系统的Mic法进行药敏试验并严格按照美国临床实验室标准化委员会(NCCLS)1999年制定的规则进行操作及判读。
METHODS To detect extended-spectrum β lactamases(( ESBLs)) by ATB microbiological system and its expert system, and compare with confirmatory test according to NCCLS. 方法采用法国生物梅里埃公司ATB细菌鉴定仪及药敏专家系统对超广谱β-内酰胺酶(ESBLs)进行检测,并用NCCLS推荐的确证试验进行比较。
Methods Susceptibility test was performed on 2 081 strains isolated from 13 hospitals, using standard plate dilution method according to the guidelines of NCCLS ( 1998). 方法按1998年美国临床实验标准委员会指导原则采用标准的平皿稀释法,对1998~1999年从13家医院分离的2081株致病菌进行敏感试验。
Methods all clinical separations fungus presses the conventional method raise appraisal, the medicine sensitive experiment is WHO the recommendation NCCLS standard method ( K-B) the law carries on. 方法所有临床分离菌均按常规方法培养鉴定,药敏试验为WHO推荐的NCCLS标准方法(K&B)法进行。
METHODS ESBLs-producing isolates were detected by the NCCLS recommended disc diffusion confirmatory test and the bacterial susceptibility test was done by K-B method. 方法按照NCCLS推荐的纸片确证法检测产ESBLs菌株,以K-B纸片扩散法进行药敏试验。
METHODS Diffusion tests were used to measure the antibiotic sensitivity of bacteria isolated from the patients with bacterial peritonitis, and resistance was analysed according to NCCLS standard. 方法用纸片扩散法对由腹腔感染患者分离的细菌进行药敏试验,并按NCCLS标准判断细菌耐药性。
METHODS The Microscan-USA was used to identify pathogens and drug sensitivity tests, the results of examination were judged according to NCCLS 1999 standard. 方法细菌鉴定采用美国Microscan微生物分析仪鉴定菌种和药敏试验,按NCCLS1999推荐方法解释结果。
Refer to NCCLS judgment standard, the results confirmed that staphylococcus aureus resistance to 9 species antibiotics. 参照NCCLS判断标准,结果证实试验所用金葡菌对9种抗菌药均表现耐药。
By the critical concentration as a standard of the United States Committee for Clinical Laboratory Standards ( NCCLS), we used microdilution method to determine the resistance of Streptococcus suis against three kinds of tetracycline, which were doxycycline, tetracycline hydrochloride and chlortetracycline hydrochloride. 以美国临床检验标准委员会(NCCLS)的临界浓度做为判断标准,采用微量稀释法判定了猪链球菌对强力霉素、盐酸四环素、盐酸金霉素3种四环素类抗生素的耐药性。