Objective: To reduce acetate accumulation in the culture of Escherichia coli by utilizing phosphotransacetylase ( PTA) mutant, so as to promote foreign protein expression and facilitate high cell density cultivation. 目的:利用大肠杆菌磷酸转乙酰基酶(PTA)缺陷变株减少工程菌乙酸的产生,以利于工程菌高密度培养及外源蛋白表达。
Influence of phosphotransacetylase on potency determination of coenzyme A 磷酸转乙酰化酶对辅酶A效价测定的影响