Investigation and clinical significance of the micrometastasis of prelaryngeal lymph nodes in the patients with glottic carcinoma 逆转录聚合酶链反应对声门癌喉前淋巴结微转移的检测及临床意义
Method: In prelaryngeal lymph nodes from 26 patients with glottic carcinoma, cytokeratin 19 ( CK19) mRNA was detected with reverse transcription polymerase chain reaction ( RT-PCR), comparing with the routine histopathologic methods ( HE staining). 方法:对26例声门癌患者的喉前淋巴结采用RT-PCR技术进行细胞角蛋白19(CK19)mRNA检测并与常规病理方法(苏木精-伊红染色)进行比较。