This paper describe the formation, isolation, denaturation and renaturation of recombinant proteins as inclusion bodies in E. coli, and summarize the most efficient ways to refold recombinant proteins. 描述了大肠杆菌异源重组蛋白质的形成、制备、变性和复性,综述了国内外变性、复性的有效方法。
AIM: To purify and refold the inclusion body of a human anti-HBsAg scFv with a 6 × His tag, and to determine the affinity constant of the purified recombinant product. 目的:对融合6×His标签的人源抗乙型肝炎表面抗原单链抗体的包涵体进行纯化和复性,并对复性产物的抗原结合性质进行鉴定。
AIM: To refold recombinant human bone morphogenetic protein-2 mature peptide ( rhBMP-2m) by different methods, and compare the activity of inducing the formation of ectopic bone. 目的:用三种不同方法复性重组人骨形成蛋白2成熟肽(rhBMP-2m),比较其异位诱骨活性。
Objective To construct mouse granulocyte-macrophage colony-stimulating factor ( mGM-CSF) expression vector and express, purify and refold mGM-CSF protein. 目的构建小鼠粒细胞-巨噬细胞集落刺激因子(mGM-CSF)工程菌,通过摸索其变、复性和纯化条件,得到高纯度高比活的重组mGM-CSF蛋白。
The purified recombinant proteins GST-dE, GST-dEM and P32-dEM were gained through denaturation and refold of inclusion bodies, affinity chromatography on glutathione-agarose column, and gel filtration chromatography on Sephacryl S-100 HR on column. 进一步通过包涵体变性复性、分子筛凝胶层析、GST亲和层析获得纯化的重组表达蛋白GST-dE、GST-dEM和P32-dEM。
In addition, the urea gradient SEC was used to refold the reduced-denatured insulin. 此外,使用脲梯度法对还原变性Ins进行了折叠研究。
Moreover, a large amount of light and Fd expression products were prepared. And then, they were mixed with equal Molar of quantity to refold into Fab by gradient dialysis. 大量制备表达的Fd及轻链后,按二者绝对量等摩尔比混合后进行透析复性。