The effects of resibufogenin on DNA synthesis and expression of correlative proteins in hepatoma cell apoptosis was observed by means of Western blotting and cytochemical method. 采用细胞免疫细胞化学显色检测和WESTERNBLOTTING分析凋亡相关基因蛋白的表达来研究脂蟾毒配基对人肝癌细胞(Bel7402)凋亡的诱导作用。
AIM: To observe the intervention of resibufogenin, one of main components of Chinese herb venenum bufonis, on the growth of human gastric cancer BGC-823 cell line cell cultured in vitro, and the relationship with cytochrome C and Caspase-3 activation. 目的:观察中药蟾酥主要成分之一&脂蟾毒配基对体外培养人胃癌BGC-823细胞系细胞生长的干预及其与细胞色素C和半胱氨酸天冬氨酸蛋白酶3活化的关系。
Influence of resibufogenin on hemodynamics in rabbits 脂蟾毒甙对家兔血液动力学的影响
Resibufogenin could induce the decrease of cell mitochondria membrane potential, increase cytochrome C in kytoplasm, enhance Caspase 3 activation, inhibit the expression of Bcl-2 protein and result in cell apoptosis. 脂蟾毒配基引起细胞线粒体膜电位下降,释放入胞质中的细胞色素C增多,促进半胱氨酸天冬氨酸蛋白酶3蛋白活化,抑制Bcl-2蛋白表达,诱导细胞凋亡。
Interventional effect of resibufogenin on the cell growth of human gastric cancer BGC-823 cell line 脂蟾毒配基对人胃癌BGC-823细胞系细胞生长的干预效应
The Study of Resibufogenin on Induction of the Apoptosis of Human Hepatocarcinoma Bel-7402 Cells by the Mitochondrial-dependent Pathway 脂蟾毒配基通过线粒体途径诱导人肝癌Bel-7402细胞凋亡的研究
With the prolongation of time and increase of concentration of resibufogenin, fluorescence intensity of cancer cell nucleus reduced and the content of cell nucleus DNA decreased obviously. 随脂蟾毒配基作用浓度和时间的延长,癌细胞核荧光强度减弱,细胞核DNA含量明显降低。
Purpose To establish a method for the determination of resibufogenin in Liushen pills by ion suppression chromatography. 目的建立测定六神丸中脂蟾毒配基的离子抑制色谱分析法。