Objective: To compare the influences of retrorsine on hepatocytes proliferation in mice and rats after liver injury, so as to investigate the feasibility of using retrorsine for establishment of liver cell transplantation model in mice. 目的:比较倒千里光碱(retrorsine)对小鼠与大鼠肝损伤后肝细胞增殖的影响,探讨应用retrorsine建立小鼠肝细胞移植模型的可行性。
Conclusion Immortalized hepatocytes of SD rat have the normal cell ultrastructure and albumin-excretion function, and retrorsine can promote the proliferation of the transplanted hepatocytes. 结论脾内移植永生化肝细胞具有正常肝细胞的形态、结构及白蛋白表达功能;Rts可促进永生化肝细胞SD大鼠脾脏内移植的增殖效率。
OVC or another kind of small hepatocyte appear only when the division of mature hepatocytes is prohibited by suppressors ( e.g., Retrorsine, RS) in advance and when acute liver injury provokes violent regeneration demand. 在使用肝细胞增殖分裂的抑制剂(例如倒千里光碱,RS)结合急性肝损伤的条件下有OVC出现,但也有人报道此时是由肝小叶内小肝细胞完成肝再生的;
Conclusion: Retrorsine can obviously inhibit hepatocyte proliferation after liver injury and is suitable for liver cell transplantation in rats, while it is the contrary in mice. 结论:应用retrorsine可明显抑制大鼠肝损伤后肝细胞增殖,适用于建立大鼠肝细胞移植模型;retrorsine对小鼠肝损伤后肝细胞增殖无明显抑制作用,不适用于建立小鼠肝细胞移植模型。