Apoptosis induced by a hammerhead ribozyme targeting the template region of telomerase RNA in NPC CNE-2Z cell line 抗端粒酶模板区核酶诱导鼻咽癌细胞凋亡的实验研究
Objective To detect the cleavage activity of Giardia canis virus ( GCV) transfer vector-mediated hammerhead ribozyme for KRR1 in vitro transcript. 目的检测犬贾第虫病毒介导的锤头状核酶对犬贾第虫滋养体核仁功能性蛋白KRR1基因体外转录体切割效率。
High-level transcription of human mitochondrial tRNA~ ( Trp) based on ribozyme and characterization on its aminoacylation activity 基于核酶技术的人线粒体色氨酸tRNA的高效转录及其活性鉴定
Inhibitory effects of proliferating cell nuclear antigen ribozyme on proliferation of retinal pigment epithelial cells in rabbits 增生细胞核抗原核酶对兔视网膜色素上皮细胞增生的抑制作用
Construction of TV Hammerhead Ribozyme Mosaicism Virus Transfer Vector FRzS 阴道毛滴虫核酶嵌合型病毒载体FRzS的构建
Research of cleavage activity of hammerhead ribozyme targeting caspase-7 in primary cultured liver cells 抗Caspase-7锤头状核酶在原代肝细胞内的切割活性研究
Effects of Telomerase Antisense RNA and bcl-2 Ribozyme on Hepatocarcinoma Cell Telomerase Activity Expression and Apoptosis 端粒酶反义RNA与bcl-2核酶对肝癌细胞端粒酶活性表达及细胞凋亡的影响
The new methods also include antisense nucleic acid technique and ribozyme technique in gene therapy. 新发展的还有反义疗法以及应用核酶进行基因治疗等。
Regulation of VEGF Expression by VEGF Specific Ribozyme in Hela Cells VEGF特异性核酶对Hela细胞VEGF表达的调节
In this article, we review the promoters and transcription complex which are dependent on RNA polymerase ⅲ. The application of RNA polymerase ⅲ-catalyzed transcription in gene therapy and gene expression by the ribozyme, antisense oligonucleotides and RNA interference is also discussed. 文章综述了依赖于DNA的RNA聚合酶Ⅲ的启动子和转录复合物,并介绍了RNA聚合酶Ⅲ的转录在基因表达和基因治疗(核酶、反义核酸、RNA干扰技术等)研究中的应用价值。
Hammerhead ribozyme consists of 3 short helices and a conserved joint chain. 锤头型核酶由3个短螺旋和1个广义保守的连接序列组成;
Inhibition of avian influenza virus replication by retrovirus-mediated specific antisense ran and ribozyme 逆转录病毒介导的特异性反义RNA和核酶抑制禽流感病毒复制的研究
Advances on Study of Ribozyme in Tumor Gene Therapy Application 核酶在肿瘤基因治疗中应用的研究进展
Gene therapy threw light on the inhibition of HCV infection, and the favorite point is ribozyme. 基因治疗为HCV感染带来了希望。其中研究的热点之一是核酶用于抗病毒治疗。
Effect of ribozyme on TIMP-1 expression and proliferation fibroblasts in human skin scars 核酶对人皮肤瘢痕成纤维细胞增殖和TIMP-1表达的影响
The cleavage reaction was conducted by mixing ribozyme and its target mRNA in a buffer containing Mg 2+. 在含有Mg2+的反应体系中,进行核酶对靶mRNA的体外切割反应。
The recombinant plasmid with the hammerhead ribozyme gene was correct by digestion identification. 核酶基因重组子经酶切鉴定序列正确。
Conclusions Computer assisted design is indispensable in studying ribozyme. 结论计算机辅助设计是研究核酶过程中不可或缺的重要步骤;
Methods Design the ribozyme cDNA fragment and construct recombinants. 方法设计核酶cDNA序列。
Ribozyme expression was confirmed by RNA dot blot; RNA斑点杂交检测核酶基因在卵巢癌细胞中的表达;
Antisense oligonucleotide ( ASON) includes antisense RNA, antisense DNA and Ribozyme. 反义寡核苷酸包括反义RNA、反义DNA和Ribozyme。
The results showed that the transgenic silkworm strain of anti NPV ribozyme has been got. 研究结果表明已经获得了抗NPV的核酶转基因蚕。
The design of HDV ribozyme and its inhibition on telomerase activity 针对端粒酶的HDV核酶的设计及其对端粒酶活性抑制研究
Objective To explore whether telomerase ribozyme could inhibit the growth of nude mice transplantation tumor. 目的探讨端粒酶核酶对裸鼠移植瘤生长的影响。
We discussed the mechanisms of ribozyme, gene silencing and antisense RNA in plant protection through the way of gene transformation. 我们利用基因转化方法,对核酶、基因沉默和反义RNA技术在抗病中的应用进行探讨。
The gene suppression effect of antisense technology and ribozyme technology is very short and weak, which is not suitable for long-term study of gene function. 反义技术和核酶技术的基因抑制效果十分短暂、微弱,不适合长期研究基因功能。