The SNV proviral cDNA clone plasmid was sequenced, the infectious clone and its complete sequence of SNV strain would be useful for further study of REV at the molecular level. 对该全基因组cDNA克隆进行酶切所获得的数个亚克隆进行测序,并将序列进行拼接,完成了REV全基因组序列。REV的这个传染性克隆将有助于进一步研究REV的分子生物学特性。
First derivative combined with standard normal variate was used to pretreat spectra, and calibration model for ginsenosides had been built by partial least squares regression method. 标准正则变换(SNV)结合一阶导数对光谱进行预处理后,采用偏最小二乘回归算法建立在线测量总皂苷浓度的近红外光谱校正模型。
The four preprocessing methods all can simplify calibration model but can't improve the prediction ability of model dramatically for the ingredient of high concentration and high absorbance, so we may choose MSC or SNV as the preprocessing method because their algorithms are much simpler than the others. 对于含量高、吸收强的组分,各种预处理方法均能简化定标模型,但不能明显提高模型的预测能力,因此可以选择算法简单的MSC或SNV进行光谱预处理;
Chicken embryo fibroblast cells ( CEF) were transfected with avian reticuloendotheliosis virus ( REV) proviral cDNA clone mixed with lipofectamine. 利用脂质体转染技术,将含有SNV株禽网状内皮组织增生症病毒(REV)前病毒全基因组cDNA克隆质粒转染鸡胚成纤维细胞(CEF)。
It turned out that: The results of all kinds of analysis for MSC and SNV are very similar, which can give an explanation theoretically why the calibration models developed by the two kinds of corrected spectra are almost the same. 结果表明,MSC和SNV校正谱的各项分析结果非常相似,从而在理论上解释了二者建立的定标模型相似性的原因;