subcultured
英
美
(subculture 的过去分词) n. 再次培养, 亚文化群
[医] 次代培养物, 次培养物
subculture 的过去分词
双语例句
- An efficient plant regeneration system was established through optimization of MS with different hormone combination and different type of root from seed and subcultured seedling in Chinese chive.
以韭菜种子萌发后根尖及继代组培苗根尖材料为外植体，以MS为基本培养基附加不同种类和浓度的植物激素诱导产生愈伤组织及芽。 - Methods Protocorm-like bodies and callus of DCWL subcultured for30 days was used as the explants, N6 was used as the basic culture with phytohormone added, and fungal extracts as the elicitor.
取继代培养30d的铁皮石斛类原球茎作接种材料，以N6为基本培养基，加入植物激素，并以真菌提取物作诱导子，对类原球茎和愈伤组织进行诱导培养。 - At the same subcultured medium and at the same subcultured period, the effect of cultural duration on the variation of calli was not significant.
在相同继代培养基和相同继代周期的条件下，培养时间对愈伤组织变异大小影响不显著。 - Stock cultures can be subcultured for working cultures weekly, typically kept as slants.
备用种可以每周接出工作种，通常工作种以斜面保存。 - It indicated that the shoot explants subcultured in vitro for10 times were suitable for shoot multiplication, and its young leaves were the better materials used for transformation.
说明继代10次的芽苗较适合用于八棱海棠离体培养的外殖体材料，其幼叶较适于遗传转化研究。 - A microbial strain cited and identified by its ATCC catalog number shall be used directly or, if subcultured, shall be used not more than five passages removed from the original strain.
通过ATCC的目录号引用和鉴定的微生物菌种应该直接使用，如果再次培养，不能从原菌群中移去超过五个菌落数。 - The growth and apoptosis of primary cells and subcultured cells were investigated by flow cytometry methods.
用流式细胞术等方法分析转基因后的原代细胞和传代细胞的生长与凋亡情况。 - Rat triceps brachii muscle was acquired to separate skeletal muscle satellite cells with the two-step method of collegenase-1 and trypsin and were cultured and subcultured in vitro.
取大鼠肱三头肌，采用二步消化法分离骨骼肌卫星细胞，体外进行原代培养和传代培养。 - Methods: The primary culture of normal rabbit bladder transitional epithelial cells were initiated by using explant culture method and the cells were subcultured in serum-free media.
方法：采用无血清培养系统，以组织块法原代培养兔正常膀胱移行上皮细胞并传代。 - The results indicated that the growth rate of cells subcultured at exponential stage was1.6 times of that at stationary stage and there was no significant difference in the taxol content.
结果表明在细胞生长对数期继代比静止期继代细胞增长率提高1.6倍，而紫杉醇产量差异不大。 - After the cells were subcultured, the frozen storage didn't influence their growth, morphology, and genetic characteristics.
通过冻存后复苏的细胞继续培养后发现，冻存对其生长、形态、遗传学等特征无明显影响。 - The effect of hormone, subculture media, desiccation treatment and supplements on regeneration of mature embryo-derived callus subcultured for a long time in rice were studied.
以长时间继代的水稻成熟胚愈伤组织为试验材料，研究激素配比、继代方式、干燥处理和添加物对分化的影响。