Thrombopoietic effect of recombinant human thrombopoietin gene transferred to mice mediated by electric pulse on normal and experimental thrombocytopenia mice 电脉冲介导的Tpo基因转移对正常及实验性血小板减少小鼠的促血小板生成作用
The in vivo thrombopoietic activity was studied by subcutaneous administration of rhIL-6 to Balb/ C mice every day for 7 days, and blood was taken from tail vein for platelet counting. 将rhIL-6和ss-PEG-rhIL-6每天给Balb/C小鼠皮下注射持续7天,每天尾静脉取血,通过计数血小板来观察rhIL-6和ss-PEG-rhIL-6促血小板增殖作用。
Objective To study the distribution characteristics of a new thrombopoietic factor in vivo in mice. 目的探讨本所研制的新型升血小板因子在小鼠体内分布情况,明确其是否具有靶向性分布的特点。
No antibody was found in all mice by ELISA. Conclusion The new thrombopoietic factor has no immunogenicity in BALB/ c mouse. 结论用新型升血小板因子免疫Balb/c小鼠,未发现能够刺激小鼠产生抗新型升血小板因子抗体。
Ss-PEG modification can enhance the half-life and stability of rhIL-6. ss-PEG-rhIL-6 may be a potential candidate for thrombopoietic agent. ss-PEG修饰可提高rhIL-6的半衰期和稳定性,ss-PEG-rhIL-6是一种很有潜力的促血小板增殖药物。
Study on immunogenicity of a new thrombopoietic factor 新型升血小板因子的免疫原性实验研究
Experimental study on targeting distribution characteristic of a new thrombopoietic factor in mice 新型升血小板因子静脉注射在小鼠体内的靶向性实验研究