transfect

英 美

网络  转染; 转入; 基因转染; 使转染; 细胞转染

过去式：transfected过去分词：transfected现在分词：transfecting

BNC.31817

双语例句

  1. From a technology perspective, their technique to transfect the estrogen receptor is an exciting advance for researching steroid receptors in the brain.
    从技术应用远景来看，他们导入雌激素受体的技术对于研究大脑中甾体激素受体功能是一大进步。
  2. Polymeric carrier containing folic acid is expected to be able to identify tumor surface receptors and transfect cells by receptor-mediated endocytosis.
    含有叶酸的聚合载体能够识别肿瘤表面受体，并且能够通过受体介导的（细胞）内摄作用转染细胞。
  3. The aim of gene therapy is to transfect DNA into target cells, in which utilizing antisense oligonucleotides is a main method.
    基因治疗是将DNA转染进入目的细胞，修复遗传错误或产生治疗因子。反义寡核苷酸的应用是基因治疗的主要手段之一。
  4. "You can transfect a gene into a fibroblast," he said.
    他说“你可以将基因转染进成纤维细胞”。
  5. Liposome Mediated Green Fluorescent Protein Transfect into Human Endothelial Progenitor Cells Derived from Cord Blood
    脂质体介导绿色荧光蛋白转染人脐血内皮祖细胞
  6. Study on Transfect Sheep Fibroblasts by DNA with GFP
    带有绿色荧光蛋白外源DNA转染绵羊成纤维细胞的研究
  7. This is the only example we know of where it is possible to image activated endogenous gene expression without having to transfect cells.
    只是激活内源性基因表达而不是转染细胞就可以使细胞成像，这个是我们所知道的惟一例子。
  8. Transfect COS-7 cells with pHII for transient expression.
    将pHII转染COS-7细胞，进行瞬时表达。
  9. Objective: The aim was to construct the recombinant of neuro related cell adhesion molecule ( NrCAM) and immunoglobin Fc fragment and transfect it into insect cells.
    目的：构建神经原相关的细胞粘附分子（NrCAM）和免疫球蛋白Fc片段融合基因的重组质粒并通过Baculovirus载体转染到昆虫细胞。
  10. Objective: To stably transfect imidazoline-1 receptor ( I 1R) in Chinese hamster ovary ( CHO) cells.
    目的：在中国仓鼠卵巢（CHO）细胞上稳定转染咪唑啉1受体。
  11. The recombined adenovirus particles containing the MOMP gene were extracted and used to transfect AD-293 cells.
    提取同源重组后的腺病毒粒子转染AD-293细胞，待重组腺病毒在AD-293细胞中传代稳定后，经PCR技术检测，扩增到了MOMP目的基因；
  12. Gene transfection is a technology that heterologous genes transfect eukaryotic cells, this article will summarize the significance, classification, methods and application of transfection.
    基因转染技术是将外源基因转染真核细胞的一种技术，本文将对外源基因转染真核细胞的目的意义、转染技术分类、方法及其应用等做一综述。
  13. CONCLUSION: Adenovirus-mediated GFP can effectively transfect the chondrocyte cultured in vitro. The tissue-engineered cartilage tissues in vivo originate from exogenous chondrocytes.
    结论：腺病毒介导的GFP能有效转染体外培养软骨细胞，外源性软骨细胞是体内组织工程化软骨组织形成的源细胞。
  14. Conclusion: jetPEI-RGD can efficiently transfect ASODN for inhibiting proliferation effect and inducing apoptosis on caco-2 cells in vitro.
    结论：jetPEI-RGD介导的bcl-2反义核酸能抑制caco-2细胞增殖和诱导细胞凋亡。
  15. Cationic liposome mediated NT-3 gene to transfect mice spiral ganglion cells
    阳离子脂质体介导小鼠原代螺旋神经节细胞NT-3基因转染的实验研究
  16. Vaccine virus could transfect DNA of CEA into DC.
    痘苗病毒可将人CEA抗原的DNA转染DC，构建针对CEA阳性肿瘤的DC疫苗。
  17. Objective: To transfect rat submandibular gland epithelial cells with plasmid vectors and develop immortalized epithelial cells.
    目的：转染质粒pSV2neo、pSV3neo，使大鼠颌下腺上皮细胞永生化，进而建立大鼠颌下腺肌上皮细胞系。
  18. Methods Transfect cos-7 cells with recombinant plasmid pVAX/ G2 and detect the transiently expressed product by IFA.
    方法重组质粒pVAX/G2转染cos-7细胞，用间接免疫荧光法（IFA）检测瞬时表达产物。
  19. Objective: To construct expression plasmids of sense and antisense cyclin D3 gene and transfect them into Raji cell line to study the biological effects of cyclin D3 gene in lymphoma cells.
    目的：构建人类cyClinD3正义和反义真核表达质粒，分别转染到淋巴瘤细胞系Raji细胞中，为探讨cyClinD3基因对淋巴瘤细胞生物学行为的影响提供研究基础。
  20. Objective Using a recombinant adenoviral vector carrying the human BMP-2 gene ( Ad-BMP-2) to transfect rabbit bone marrow stromal cells ( BMSC) to study the effects of Ad-BMP-2 transfection on cell differentiation.
    目的：用人骨形态发生蛋白2腺病毒表达载体（Ad-BMP-2）转染兔骨髓基质干细胞（BMSC），观察Ad-BMP-2转染对细胞分化的影响。
  21. Methods Transiently transfect COS-7 cells with expression vector pBT-2s of recombinant human dimeric erythropoietin and identify by dot blot.
    方法用重组人红细胞生成素二聚体表达载体转染COS-7细胞，通过dotblot进行检定。
  22. The correctly cloned plasmids were subjected to rapid in vitro transcription and translation identification. Liposome mediated, recombinant plasmids were used to transfect human endothelial cell strain and the products were identified.
    对重组质粒进行酶切鉴定和测序分析，将正确克隆的质粒分别作体外快速转录翻译鉴定，以及经脂质体介导转染人内皮细胞株，并对表达产物进行鉴定。
  23. Phospholipid-coated microbubbles have a good histocompatibility and high gene transfect efficiency.
    研究发现脂质微泡具有良好的组织相容性、高效的基因传输效果及柔韧易于表面构建的外壳，使它可能成为最有希望的靶向治疗有效载体。
  24. We transfect these plasmids into cells and all the viral coding proteins were detected by western blotting.
    用HCoV-229E的表达质粒转染细胞后通过蛋白免疫印迹可以检测到各蛋白的表达。
  25. The complexes can transfect cells in serum containing environment. 3.
    这种复合物的转染过程可在含血清环境中进行。
  26. So we used the eukaryotic expression vector to transfect them to PC-3 cells and examined the expression of this two proteins by RT-PCR and immunofluorescence assay. And we detected the activity of them by MTT assay and cell apoptotic assay in the last.
    为此我们更换表达载体，应用真核表达载体，转染PC-3细胞，表达目的蛋白；RT-PCR、细胞免疫荧光法检测目的蛋白的表达；最后分别用MTT法和细胞凋亡测定表达产物水解酶活性。
  27. Then vectors were used to transfect T3 cells by liposome 2000 and virus titer was tested.
    以脂质体2000转染T3细胞，测定病毒滴度。
  28. The recombinant plasmid pXL/ EG ⅳ/ GFP/ Neo, which is extracted using a endotoxin-free plasmid extracting kit, was used to transfect silkworm BmN cells, and the result showed that the GFP and Neo were expressed successfully.
    用去内毒素的质粒抽提试剂盒提取重组质粒pXL/EGⅣ/GFP/Neo后，转染家蚕BmN细胞，确认GFP和Neo的表达正常。
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