Chloroplast transformation, an alternative way to incorporate foreign genes into plant genome, has a number of advantages: higher transgene expression level; 叶绿体转化系统,独立于传统的核转化,为植物导入外源基因提供了新途径。它有如下优点:超量表达目的基因;
Construction of the Insect-resistant Gene Vector and Study on the Transgene of Chinese Cabbage 抗虫基因载体的构建及白菜转基因研究
Objective: To investigate the information of transgene integrations in mice mediated with lentiviral vectors, the inverse PCR was used to clone the sequences of integration sites. 目的:为分析慢病毒介导的转基因小鼠中外源基因整合位点的信息,应用反向PCR克隆整合位点序列。
A Study of Expression of Growth Factors in the Early Tendon Healing Period and Delivery of Transgene for Tendon Repairs 生长因子在肌腱损伤愈合早期中的表达变化及转基因治疗肌腱损伤的初步研究
But on the other hand, the transgene zoology and botany is in itself also hiding many risks, from this initiates the malignant event is not rare. 但是另一方面,转基因动植物本身也隐藏代写毕业论文着很多风险,由此而引发的恶性事件并不少见。
The Study of Isolation, Culture and Transgene of Porcine Spermatogonial Stem Cells 猪精原干细胞的分离、培养及转基因条件研究
The transgene of choice is then injected into a fertilized egg, which is obtained as follows; A female mouse is injected with several hormones to cause it to produce numerous eggs; 然后将这段基因注入受精卵,方法如下:对一只雌老鼠注射激素使其产生大量卵;
Single Colony Preparation and Amplification Culture of Fetal Fibroblast and Mammary Gland Epithelial Cell of Human Lactoferrin Transgene from Goat 山羊转人乳铁蛋白基因成纤维细胞和乳腺上皮细胞单克隆的制备及扩大培养
The transgene results were confirmed by fluoroscopy and the cell viability after transfection was evaluated by light microscopy and electron microscopy. 结果:经荧光显微镜下观察证实:成功地对骨髓间充质干细胞实现了基因转染。
Advances of Research on Cotton Transgene Technology and Its Target Characters 棉花转基因技术及目标性状研究进展
Application of Transgene Stacking in Breeding of New Cotton Varieties 转基因堆积在棉花新品种选育中的应用
An Experiment Study on Transgene Expression in Intervertebral Disc Cells in Vivo Mediated by Microbubble-Enhanced Ultrasound Gene Therapy 超声微泡介导双报告基因在椎间盘髓核中表达的实验研究
Copy Number and Integration Site of Transgene in Transgenic Pig 转基因猪中外源基因拷贝数和整合位点的研究
With the continuous development of this technology and being on the market of its variety, we pay more and more attention to the safety of transgene variety. 认为随着转基因技术的不断发展和转基因品种的逐渐面市,转基因产品的安全性问题越来越引起人们的关注。
Positional Effects of Matrix Attachment Regions on Transgene Expressions 核基质结合区的位置对转基因表达的影响
The Study of Enhancement of Recombinant Adeno-associated Virus Transgene to Myocardium by Targeted Echo-contrast Agent 超声造影剂介导下增强腺病毒相关病毒心肌靶向转染率的实验研究
There are four main points: 1.Using tissue-specific promoters ( TSPs) to direct transgene expression to target tissues for reducing adverse effects in non-target tissues; 主要体现在:1、由组织特异性启动子参与调控,增强基因转染的靶向性,降低非肿瘤组织的副作用;
For a successful gene therapy-based treatment, efficient delivery of a transgene to target neurons is also essential. 对一种成功的基因疗法而言,能有效地将转基因送达靶向神经元是十分重要的。
Evolutionary theory of hybridization-introgression: its implication in environmental risk assessment and research of transgene escape 杂交-渐渗进化理论在转基因逃逸及其环境风险评价和研究中的意义
Study of Rapid Detection of Transgene Sequences in Cottonseed Oil Using PCR 转基因棉子油的快速PCR检测研究
These results suggested that the existence of AAV ITRs played an active role for long-term transgene expression even without tans elements. 这一结果提示我们,在离体条件下,即使没有反式因子的参与,AAVITR序列的存在仍然对转基因的长期表达有着积极的作用。
In the course of commercialization plant gene engineering, transgene silencing is one of the obstacles. 转基因植物中转基因沉默已成为植物基因工程的一大障碍。
Mechanism and Functional Analysis of a Tobacco MAR on Stability of Transgene Expression 烟草核基质结合序列在转基因遗传稳定性中的功能及机理分析
The functional strength was correlated with the level of transgene expression. 其功能的强弱与其表达量相关。
Experimental Study of Intralipid Effects on Transgene Expression with an Recombinant Adenoviral Vector Expressing Interleukin 12 in Vivo Intralipid对重组鼠IL-12腺病毒转基因表达影响的体内实验研究
Analysis the Mechanism of Antisense Transgene Mediated Gene Silencing; 反义转基因介导的基因沉默机制的研究(分析)
However, due to the existing in human embryos high rates of chromosome chimeric type, single transgene ball whether can represent the entire embryo also requires people to further study. 然而,由于人类胚胎中存在高比例的染色体嵌合型,单个卵裂球是否能代表整个胚胎还需要人们进一步研究。
With the development of genetic engineering, more and more people pay attention to transgene silencing. 随着转基因技术在各个领域的深入发展,转基因沉默已引起越来越多的人关注。
DNA methylation? repeat sequence? trans-inactivation and co-suppression are the most important reasons what induce transgene silencing. 甲基化、重复序列、反式失活和共抑制是基因沉默的主要诱因。
Clone and Prokaryotic Expression of Plant Defensins Genes from Cruciferae and Transgene to Arabidopsis 十字花科植物防御素基因克隆和原核表达及转拟南芥研究