Construction of recombinant adenovirus vector with TRX gene by Tet-Off induction Tet-Off诱导的硫氧还蛋白基因重组腺病毒载体的构建
Effect of Anti-TRX s Gene on Thioredoxin Reduction Characteristics in Seeds of Transgenic Wheat 反义TrxS基因对转基因小麦籽粒硫氧还蛋白还原活性的影响
All in all, the Trx h activity has been greatly enhanced and it has caused the higher activities of amylase, quick metabolism of carbon and strong seedlings-vigor in transgenic beer barley seeds. 总的来看,转基因大麦的Trxh活性得到了很大提高,而Trxh活性的变化又进一步提高了啤酒大麦籽粒中的淀粉酶活性,促进了碳代谢的进程,提高了籽粒发芽势。
Trx plays crucial roles in regulating cell growth, apoptosis and gene transcription. Trx具有调节细胞生长、抑制凋亡、调节基因转录等功能。
HPO could interact with TRX in vitro and in vivo, which is the critical cytokine in redox regulation. 体、内外实验结果表明,HPO分子和作为氧化还原开关的TRX分子在细胞内外存在直接的相互作用。
Thioredoxin ( Trx) is a small and conservative protein. 硫氧还蛋白Thioredoxin(Trx)是一类高度保守的低分子量蛋白质。
Thioredoxin ( TRX) is a low molecular weight protein found in both prokaryotic and eukaryotic cells and has a conserved Cys Gly Pro Cys active site.. It plays an important biological role as a multifunctional protein. 硫氧还蛋白(Thioredoxin,TRX)是广泛存在于原核和真核细胞中的低分子量蛋白质,它含有保守的CysGlyProCys活性位点,作为多效性细胞因子而具有重要的生物学功能。
In Escherichia coli, TGase was expressed in the form of soluble and active enzyme. TGase would be retained the biological activity even when there was another protein ( thioredoxin in this paper) was fused at its N teminal. 而在大肠杆菌系统中,TGase获得了高效的可溶性表达,且发现在TGase的N端融合了硫氧还蛋白(Trx)后仍能保持其生物学活性。
CONCLUSION: TRX protein plays an important role in the defense response of myocardium against oxidative stress. 结论:TRX蛋白在心肌的氧化应激防御反应中起重要作用。
Trx has various biological functions in keeping stable redox status of cells. 硫氧还蛋白具有多种生物学功能,对维持体内稳定的氧化还原状态具有重要的作用。
Trx system can dethiolate protein disulfides and thus is an extremely important regulator for redox homeostasis in the cells. 硫氧还蛋白系统能使蛋白质二硫化物脱硫醇,是细胞内氧化还原稳态的重要调节子。
Objective To investigate the changes and potential role of the expression of thioredoxin ( Trx) in lung of premature rats exposed to hyperoxia and its clinical significance. 目的研究高氧肺损伤早产鼠肺组织硫氧还蛋白(Trx)的表达及其临床意义。
The gene of 7-mer peptide was cloned and the expression plasmid pET-32a (+)_Trx_RBIP was constructed. 将该短肽的基因插入质粒pET-32a(+),与硫氧还蛋白(Trx)在大肠杆菌BL21(DE3)中融合表达;
Early researches on small cell lung cancer apoptosis mechanism have concluded that, FGF-2 significantly increases the survivin protein expression of the strongest inhibition of apoptosis, and TRX may participate in the FGF-2 inhibit lung cancer apoptosis of small cell. 前期对小细胞性肺癌凋亡抑制机制的研究表明,FGF-2能显著上调凋亡抑制作用最强的Survivin蛋白表达,且TRX可能参与了FGF-2抑制小细胞性肺癌凋亡作用。
In addition, whether FGF-2 and TRX expression in NSCLC have correlation, and the clinical significance of such expression of FGF-2 and TRX in non-small cell lung cancer have not been reported at home and abroad as well. 在NSCLC组织中FGF-2和TRX的表达是否有相关性以及FGF-2和TRX在非小细胞肺癌组织中表达的临床意义等目前国内外也未见文献报道。
Thioredoxin ( TRX) is a widely distributed redox protein. 硫氧还蛋白(Thioredoxin,TRX)是一个分布广泛的氧化还原蛋白。
Trx has many biological functions, such as antioxidation, resistance to apoptosis, growth factors and regulation of transcription factor activity. Trx具有多种生物学功能,如抗氧化、抗凋亡、生长因子及调节转录因子活性等。
GGA, an inducer of Trx, inhibited the ER stress induced by MPP+ in vitro. 在细胞水平,硫氧还蛋白诱导物GGA可以缓解MPP+引起的内质网应激,从而抑制细胞凋亡。
Baicalin can increase the Trx level in protein level and transcriptional regulation both in vitro and in vivo, and also have protective effect on PD rats behavior change and peroxidation. 在整体动物实验中,黄芩苷对PD大鼠行为学变化和脑内过氧化反应有一定的保护作用。黄芩苷可以在蛋白水平和转录水平提高PD大鼠脑内的Trx浓度。
Whether there is another way for FGF-2, that is through inducing the expression of TRX in non-small cell lung cancer to achieve the purpose of inhibiting tumor cell apoptosis, having not been reported at home and abroad. FGF-2是否存在另一途径即通过诱导非小细胞肺癌中TRX的表达来达到抑制肿瘤细胞凋亡的,目前国内外尚未见报道。
Redox system in plants is mainly present in the cytoplasm, chloroplasts and mitochondria, regulating the activity of target proteins via thioredoxin-mediated redox system. 植物中的氧化还原信号调节系统主要存在于细胞质、叶绿体和线粒体内,主要通过硫氧还蛋白(Trx)介导的氧化还原系统来实现对靶蛋白活性的调节。
Trx protects neuron from ER stress-mediated cytotoxicity induced by MPTP, and the molecular mechanism is still unclear. 硫氧还蛋白具有保护多巴胺能神经元免受MPTP所致内质网应激损伤,然而这一作用的分子机理还不十分清楚。
Suggesting decline of Trx in AD and the occurrence of apoptosis has important links and there may be through Cyto-C release and activation of Caspase-9 caused apoptosis. 提示AD中Trx的下降与细胞凋亡的发生之间有重要的联系,并且有可能是通过Cyto-C的释放和Caspase-9的激活而引起凋亡。
These studies have shown that: TRX can suppress tumor cell apoptosis. 研究表明:TRX可以抑制肿瘤细胞凋亡。
Thus, Trx is a good candidate for the study on the drug addiction. 因此,Trx是研究毒品成瘾的一个重要的靶分子。
Thioredoxin ( Trx) is a kind of small molecule protein which is expressed in various organisms. 硫氧还蛋白(Trx)是一种广泛表达于多种生物的小分子蛋白质。
Thioredoxin binding protein-2 ( TBP-2) is one kind of negative regulator of Trx. 硫氧还蛋白结合蛋白2(TBP-2)则是一种Trx的负调节蛋白。