Effect of Feeder Layer Culture System and Leukemia Inhibitory Factor on Keeping Mouse Embryonic Stem Cells in Undifferentiation 饲养层细胞体系及白血病抑制因子对维持小鼠胚胎干细胞未分化状态的作用
Small molecules in maintaining embryonic stem cell undifferentiation 小分子物质对维持胚胎干细胞未分化状态的调控作用
RH-CM can be used as supplement of ES cell media. ES cells which are cultured in media containing 70% RH-CM and on PMEF feeder can maintain their undifferentiation state and diploid karyotype. RH-CM也可作为小鼠ES细胞培养基的添加物,用含70%RH-CM的ES细胞培养基和小鼠胚胎原代成纤维细胞饲养层(PMEF)培养ES细胞,可长期有效地维持其未分化状态和二倍体核型。
Principle Uncontrolled growth, the characteristic of tumor histology consist of excess proliferation and undifferentiation with embryonic characters. 1基本原理肿瘤的组织学特点是细胞生长失控、过度增殖、分化停止并呈现某些胚胎性状。
When isolated EG cells were examined by morphology observation, AKP strain and in vitro differentiation ability, they were convinced to have a series of characters of mice EG cells including cloning grow way, undifferentiation state and pluripotency of cells. 对所分离EG细胞经形态观察、AKP染色和体外分化能力检测,证实其符合小鼠EG细胞的集落状生长、细胞未分化特性及细胞多能性等特征。
No cell surface markers were detected in eight cases defined as acute undifferentiation leukemia. 有8例未测到任何标记抗原,归属于急性未分化型白血病。
Identification of undifferentiation markers on human corneal and limbal epithelia 人角膜及角膜缘上皮细胞分化标记的检测
Histological studies showed: apical meristems undifferentiation of some abnomal embryos was one of factors which affect production of complete plant. 对成熟处理过的体细胞胚的组织学研究表明,形态学上苗端未分化是形态不正常胚不能形成完整植株的原因之一。
Genes regulating development and differentiation participate in the embryo development and differentiation of inner cell mass and trophectoderm, which controls the transition from undifferentiation to differentiation stage. 调节发育和分化的基因参与胚胎的发育和内细胞团、滋养外胚层细胞的分化,调节胚胎由未分化状态向分化状态过渡。
Overexpression of the BCL 2 proto oncogene in esophageal small cell undifferentiation carcinoma BCL-2癌基因蛋白在食管小细胞未分化癌表达的研究
Conclusion It is very important for special culture medium to keep undifferentiation state in MSC culture. 结论专用培养基对MSC体外培养中保持未分化状态很关键。
The alkaline-phosphatase-positive cells can be maintained growth well and undifferentiation until 6 passages. AKP染色阳性细胞在上述培养基中传至6代仍生长良好和保持未分化状态。
EDAG gene has the activity of malignant transformation and is relative to maintaining undifferentiation of cells. 研究表明EDAG具有恶性转化活性,与维持细胞的未分化状态有关。
Embryonic stem cells ( ESCs) are pluripotent cell lines of earlier embryonic origin cultivated in vitro with anti-differentiation factors. When cultured in vitro, ESCs can keep undifferentiation state and passage to the future generation. 胚胎干细胞(ESCs)是早期胚胎经体外分化建立的多能性细胞系,体外培养时保持未分化状态,可以传代增殖。
These results indicate that feeder layer has certain action in suppressing ES cell differentiation in culture, while exogenous LIF play predominant role in maintaining ES cells undifferentiation. 4. 说明饲养层细胞在ES细胞的分化抑制中有一定作用,而起主导作用的是外源LIF。
In the junior high pupil, the bipolar type occupies majority, next is the undifferentiation type, last is the virilized type, what occupies the proportion to be least is the feminization type. 在初中生中,双性化类型占大多数,其次是未分化类型,再次是男性化类型,所占比重最少的是女性化类型。
The process could be divided into five phases: undifferentiation stage, outer petal differentiation stage, inner petal differentiation stage, stamen differentiation stage, and pistil differentiation stage. 其过程可分为未分化期、外轮花瓣分化期,内轮花瓣分化期,雄蕊分化期,雌蕊分化期五个时期。
The main resons are as follows: 1. The process of Apricot flower buds morphological differentiation can be divided into six stages: undifferentiation period, differentiation period, calyx differentiation period, petal differentiation period, stamen differentiation period and pistil differentiation period. 结果如下:(1)杏花芽形态分化过程分为:未分化期、分化初期、萼片原基分化期、花瓣原基分化期、雄蕊原基分化期和雌蕊原基分化期六个阶段。
Under transmission electron microscopy, undifferentiation P19 cells had a large nucleus and plenty of cell organ which distributed around nucleus, and there were abundance hepatin granule. 透射电镜观察未经诱导的P19细胞胞核较大,细胞器丰富分布于细胞核周围,胞质中可见大量糖原颗粒。