The use of transplant deep venose tube in the drainage and perfusion treatment of pleural effusion 移植深静脉插管术用于胸腔积液的置管引流和灌注治疗
Methods: 2 ml venose blood from chronic hepatitis patients was collected, and nourished with phytohemagglutinin ( PHA)( 20 μ g/ ml). Four hours later, the expression of CD69 was tested by flow cytometry ( FCM). 方法:静脉采集慢性乙肝患者普通肝素抗凝血2ml,在植物血凝素(PHA)20μg/ml条件下进行全血培养,4h后用双色免疫荧光标记流式细胞术检测CD4+、CD8+T淋巴细胞CD69的表达。