endonuclease

1. Restriction enzyme: Protein ( more specifically, an endonuclease) produced by bacteria that cleaves DNA at specific sites along its length.
   限制性内切：由细菌产生的一种蛋白质，能在特定的地方切断去氧核糖核酸分子。
2. Extraction and Restriction Endonuclease Analysis of Genomic DNA and Sequence Analysis of TK Gene from Goatpox Virus
   山羊痘病毒基因组提取、酶切及TK基因克隆测序分析
3. Preparation and characterization of rabbit anti-human apurinic/ apyrimidinic endonuclease ( APE1) polyclonal antibody
   兔抗人APE1多克隆抗体的制备及鉴定
4. Studies on the Active Groups of Pst I Restriction Endonuclease
   限制性核酸内切酶PstⅠ活性基团的研究
5. Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.
   方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。
6. They then introduced the homing endonuclease gene ( HEG) which makes a copy of itself, ensuring all offspring end up with a copy of it as well into around one per cent of the population.
   然后他们向这个种群的大约1%引入了归巢内切酶基因（HEG），该基因能够制作一份自我拷贝，确保所有的后代都有它自己的一份拷贝。
7. Correlation Research between Gene Polymorphism of Vitamin D Receptor Fok ⅰ Restriction Endonuclease and Susceptivity of Gastric Cancer in Han People of Shandong Province
   山东省汉族人群维生素D受体基因FokⅠ酶切位点多态性与胃癌易感性的相关性研究
8. Prokaryotic Expression and Application of Serratia marcescens Non-specific Endonuclease
   Serratiamarcescens非特异性核酸内切酶的原核表达及其应用
9. Choice of restriction endonuclease and establishment of silver-staining method in cDNA-AFLP System for Carthamus tinctorius
   红花cDNA-AFLP限制性内切酶的选择及银染体系的建立
10. Study of Preparation and Restriction Endonuclease of B. cepacia HMW DNA
    洋葱伯克霍尔德氏菌HMWDNA的制备及酶切研究
11. A Novel Fluorescence Assay for the Activity of Restriction Endonuclease Based on Molecular Beacon
    分子信标检测限制性内切酶活性的新型荧光分析方法
12. Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls;
    并对发现突变的基因进行分析。方法应用PCR扩增、DNA序列测定等技术分析所有AR基因外显子及其邻近DNA序列片段；
13. The recombinant plasmid PET-11D-ospC was identified with restriction endonuclease analysis and sequencing.
    采用酶切分析及序列测定等方法鉴定重组质粒的正确性。
14. Result The recombinant was correctly constructed and restriction endonuclease analysis and PCR amplification.
    结果经酶切鉴定及基因测序证实重组三突变型穿梭质粒构建成功。
15. Results: Recombinant plasmid was proved to carry P1 protein gene by using PCR and endonuclease map analysis method.
    结果：PCR和限制性核酸内切酶图谱分析均证实所获重组质粒中含有P1蛋白基因。
16. Successful construction of AT cloning was confirmed by PCR, restriction endonuclease fragment length polymorphism analysis and sequencing.
    限制性酶切长度多肽性分析，PCR及DNA序列测定等均证实克隆构建成功。
17. PCR products were then restricted by Apa I endonuclease.
    用限制性内切酶ApaⅠ分析扩增产物。
18. Physical states of 8 HPV 11 DNA were analyzed by techniques of restriction endonuclease and Southern blot hybridization.
    用限制性内切酶和Southern转印杂交法分析了8例HPV11DNA的物理状态。
19. Results The recombinant plasmid pAd-K14-E6/ E7-polA was successfully constructed and confirmed by restriction endonuclease digestion and sequencing.
    结果通过同源重组的方法构建了腺病毒pAd-K14-E6/E7-polA载体，经酶切和测序鉴定该质粒构建成功。
20. The amplification and endonuclease analysis of 28s rRNA gene of blood flukes
    冷血和温血动物血吸虫28SRRNA基因的扩增和酶切片段分析
21. In this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis.
    本研究利用多种组合的酶切分析，绘制了较为详细的酶切图谱。
22. The technique is useful in the analysis of restriction endonuclease digestion of genomic DNA and Southern blot.
    该法提取的血吸虫基因组DNA较纯，适合于以后进行DNA限制性核酸内切酶谱分析和Southernblot分析。
23. Methods: DNA sequencing and restriction endonuclease reaction was used.
    方法：采用DNA测序及限制性酶切反应方法。
24. Methods Plasmid profile and plasmid DNA restriction endonuclease analyses were carried out on 43 strains of S.flexneri 2a.
    方法对43株F2a志贺菌进行质粒图谱和质粒DNA酶切图谱分析。
25. Methods Cell culture and methylation sensitive restriction endonuclease digestion were used.
    方法采用细胞培养和限制性内切酶解分析技术观察分析细胞生长及细胞基因组DNA甲基化水平。
26. The recombinant fragment of pMD-T-fbps was verified by restriction endonuclease analysis and sequencing.
    经内切酶酶切和测序鉴定后，将由pMD-T-fbps内切酶切下的片段定向克隆于表达载体pET-32a（+），得到重组质粒pfbps。
27. This was confirmed by cleavage of restriction endonuclease and DNA sequencing.
    通过质粒双酶切和DNA测序证实该重组质粒构建正确。
28. Methods Plasmid profile analysis, Plasmid DNA restriction endonuclease analysis, RAPD-PCR, Set1/ set2-PCR.
    方法对43株从患者粪便和食物中分离菌株进行质粒图谱分析、质粒DNA酶切图谱分析、随机PCR分析、set1/set2毒力基因PCR分析。
29. Restriction endonuclease and target fragment PCR detection results show that the expression vector was constructed successfully.
    经过酶切鉴定和目的片段PCR检测鉴定，证明表达载体构建成功。
30. Traditional methods to assay the activity of restriction endonuclease are discontinuous and time consuming.
    传统的检测限制性内切酶活性的方法是不连续的，费时的。

noun

1. a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes